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liver development, regeneration and disease. Trends Genet.
None. 2020;36(8):587-597.
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Funding
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This research was supported by the National Natural Science Multimodal decoding of human liver regeneration. Nature.
Foundation of China (62127811, U23A20453, U21A20396, 2024;630(8015):158-165.
T2222029), the National Key Research and Development doi: 10.1038/s41586-024-07376-2
Program of China (2022YFA1104701), Initiative Scientific 6. Sun T, Pikiolek M, Orsini V, et al. AXIN2<sup>+</sup>
Research Program of Institute of Zoology (2023IOZ0101), Pericentral hepatocytes have limited contributions to liver
and the CAS Engineering Laboratory for Intelligent Organ homeostasis and regeneration. Cell Stem Cell. 2020;26(1):97-
Manufacturing (KFJ-PTXM-039). 107.e6.
doi: 10.1016/j.stem.2019.10.011
Conflict of interest 7. Raasch M, Fritsche E, Kurtz A, Bauer M, Mosig AS.
Microphysiological systems meet hiPSC technology -
The authors declare no conflicts of interest. new tools for disease modeling of liver infections in basic
research and drug development. Adv Drug Deliv Rev. 2019;
Author contributions 140:51-67.
Conceptualization: Yifan Hou, Xin Liu, Shen Ji, doi: 10.1016/j.addr.2018.06.008
Xinhuan Wang 8. Heymann F, Tacke F. Immunology in the liver — from
Investigation: Yifan Hou homeostasis to disease. Nat Rev Gastroenterol Hepatol.
Supervision: Qi Gu 2016;13(2):88-110.
Visualization: Yifan Hou doi: 10.1038/nrgastro.2015.200
Writing – original draft: Yifan Hou, Xin Liu 9. Palakkan AA, Hay DC, PR Anil Kumar, TV Kumary, Ross
Writing – review & editing: Yifan Hou, Xin Liu, Shen Ji, JA. Liver tissue engineering and cell sources: issues and
Xinhuan Wang, Qi Gu challenges. Liver Int. 2013;33(5):666-676.
doi: 10.1111/liv.12134
Ethics approval and consent to participate 10. Prior N, Inacio P, Huch M. Liver organoids: from basic
research to therapeutic applications. Gut. 2019;68(12):
Not applicable.
2228-2237.
doi: 10.1136/gutjnl-2019-319256
Consent for publication
11. Osonoi S, Takebe T. Organoid-guided precision hepatology
Not applicable. for metabolic liver disease. J Hepatol. 2024;80(5):
805-821.
Availability of data doi: 10.1016/j.jhep.2024.01.002
Not applicable. 12. Panwar A, Das P, Tan LP. 3D hepatic organoid-based
advancements in LIVER tissue engineering. Bioengineering
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Volume 10 Issue 5 (2024) 143 doi: 10.36922/ijb.3819

