Page 218 - IJB-10-5

International Journal of Bioprinting Biomimetic osteochondral scaffold

Figure 7. Osteogenic differentiation of rat bone marrow mesenchymal Figure 8. Chondrogenic differentiation of rat bone marrow mesenchymal
stem cells (rBMSCs) in the integrated osteochondral scaffolds. (A–C) stem cell (rBMSC) microspheres in the integrated osteochondral tissue
Immunofluorescence staining of F-actin (red), osteogenic biomarkers engineering scaffolds. (A–C) Immunofluorescence staining of F-actin
(RUNX2, COL I, and OCN) (green), and DAPI (blue) at the subchondral (red), chondrogenic biomarkers (SOX9, COL II, and ACAN) (green),
or cartilage layer of the integrated osteochondral scaffolds. (D) and DAPI (blue) at the cartilage or subchondral layer of the integrated
Expression of osteogenic genes, including OCN, COL I, and RUNX2, osteochondral scaffolds. (D) Expression of chondrogenic genes, including
at the subchondral layer of the integrated osteochondral scaffolds. *p < SOX9, COL II, and ACAN, at the cartilage layer of the integrated
0.05; ns denotes p > 0.05. Abbreviations: BMP-2, bone morphogenetic osteochondral scaffolds. *p < 0.05. Abbreviations: ACAN, aggrecan; BMP-
protein-2; COL I, collagen I; FGF-18, fibroblast growth factor-18; OCN, 2, bone morphogenetic protein-2; COL II, collagen II; CM, chondrogenic
osteocalcin; OM, osteogenic medium; RUNX2, runt-related transcription medium; FGF-18, fibroblast growth factor-18; SOX9, sry-related HMG
factor factor-2; TCP, tissue culture plate group. box protein-9; TCP, tissue culture plate group.

Volume 10 Issue 5 (2024) 210 doi: 10.36922/ijb.3229