Page 292 - IJB-10-5

International Journal of Bioprinting 3D printing of collagen II-scaffolds

Figure 8. Microscopic images of mesenchymal stem cells (MSCs) stained with DAPI (blue) and F-actin (red) at 96 h of culture: (A and B) CNF/gelatin/
alginate (A) and CNF/collagen I/alginate (B) scaffolds with a rod distance of 450 μm; (C) CNF/collagen II/alginate scaffold in bulk nonporous form;
(D–F) CNF/collagen II/alginate scaffolds with a rod distance of 320 (D), 450 (E), and 550 μm (F), respectively; (G–H) CNF/gelatin/alginate (G) and CNF/
collagen I/alginate (H) scaffolds with a rod distance of 450 μm; (I) collagen II-based bulk nonporous scaffolds; and (J and K) morphology of cell aggregates
in CNF/collagen II/alginate mesh porous scaffold with a rod distance of 320 μm. Scale bars: 400 µm (A–F); 200 µm (G–J); 100 µm (K). Abbreviation: DAPI,
4’,6-diamidino-2-phenylindole. Magnifications: J (100×), K (200×).

Table 2. Average pore size and rod diameter of scaffolds in samples a–f
Sample no. Material and composition Methods of fabrication Scaffold structure
a 2% (w/v) CNF/ 3% (w/v) gelatin/ 15% (w/v) 3D printing Mesh scaffold, rod- distance at 450 μm
alginate
b 2% (w/v) CNF/ 3% (w/v) collagen I/ 15%
(w/v) alginate
c 2% (w/v) CNF/ 3% (w/v) collagen II/ 15% Syringe injection Nonporous and solid scaffold; the concept of
(w/v) alginate (into the culture-plate) rod-distance was not applicable
d 3D printing Mesh scaffold, rod-distance at 320 μm
e Mesh scaffold, rod-distance at 450 μm
f Mesh scaffold, rod-distance at 550 μm
Abbreviations: CNF, cellulose nanofiber; N/A, not applicable.

Volume 10 Issue 5 (2024) 284 doi: 10.36922/ijb.3371