Page 297 - IJB-10-5

International Journal of Bioprinting 3D printing of collagen II-scaffolds

applied to regenerated tissues to increase their volume and Experimental Animal Ethics Committee of Anhui Medical
enhance their mechanical properties. This approach could University (approval number: LLSC 20221081).
reduce the gap between the regenerated tissue and native
host tissue, facilitating the clinical translation of collagen Consent for publication
II-based scaffolds for tissue repair applications.
Not applicable.
5. Conclusion Availability of data

In this work, collagen II-containing mesh scaffolds were Research data are available in the Supplementary File.
successfully fabricated by 3D printing, and the scaffolds
feature a much higher resolution than most reported References
hydrogel-based scaffolds. Collagen II enhances the level of
MSC condensation based on the cell distribution pattern 1. Petitjean N, Canadas P, Royer P, Noël D, Le Floc’h S. Cartilage
and quantitative upregulation of FAK and N-cadherin. biomechanics: from the basic facts to the challenges of tissue
Therefore, collagen II-containing scaffolds exhibit greater engineering. J Biomed Mater Res A. 2023;111(7):1067-1089.
chondrogenic differentiation compared to gelatin-based doi: 10.1002/jbm.a.37478
scaffolds. Additionally, smaller pores and rod diameters 2. Xu X, Xu L, Xia J, Wen C, Liang Y, Zhang Y. Harnessing knee
were found to promote the proliferation and chondrogenic joint resident mesenchymal stem cells in cartilage tissue
differentiation of MSCs, synergistic to the enhancement engineering. Acta Biomater. 2023;168:372-387.
of collagen II. Therefore, we demonstrated the effect doi: 10.1016/j.actbio.2023.07.024
and underlying mechanism of collagen II in enhancing
chondrogenic differentiation and constructed a scaffold with 3. Stampoultzis T, Karami P, Pioletti DP. Thoughts on cartilage
optimized compositional and structural characteristics. tissue engineering: a 21st century perspective. Curr Res
Transl Med. 2021;69(3):103299.
These findings will broaden our understanding of scaffold doi: 10.1016/j.retram.2021.103299
design for cartilage tissue engineering.
4. Nguyen TPT, Li F, Shrestha S, et al. Cell-laden injectable
Acknowledgments microgels: current status and future prospects for cartilage
regeneration. Biomaterials. 2021;279:121214.
The authors would like to thank the Center for Scientific doi: 10.1016/j.biomaterials.2021.121214
Research of Anhui Medical University for their valuable 5. Yang Z, Li H, Yuan Z, et al. Endogenous cell recruitment
assistance with our experiments. strategy for articular cartilage regeneration. Acta Biomater.
2020;114:31-52.
Funding doi: 10.1016/j.actbio.2020.07.008
This work was supported by the National Natural 6. Wu Z, Korntner SH, Mullen AM, Zeugolis DI. Collagen type
Science Foundation of China (81772408) and the Key II: from biosynthesis to advanced biomaterials for cartilage
Research and Development Program of the Anhui engineering. Biomater Biosyst. 2021;4:100030.
Province (2022e07020046). doi: 10.1016/j.bbiosy.2021.100030
7. Huang H, Ayariga J, Ning H, Nyairo E, Dean D. Freeze-
Conflict of interest printing of pectin/alginate scaffolds with high resolution,

The authors declare no conflicts of interest. overhang structures and interconnected porous network.
Addit Manuf. 2021;46:102120.
Author contributions doi: 10.1016/j.addma.2021.102120
8. Statham P, Jones E, Jennings LM, Fermor HL. Reproducing
Conceptualization: Kaixuan Li, Hanxiao Huang the biomechanical environment of the chondrocyte
Formal analysis: Kaixuan Li for cartilage tissue engineering. Tissue Eng Part B Rev.
Funding acquisition: Cailiang Shen 2021;28(2):405-420.
Investigation: Kaixuan Li, Hanxiao Huang doi: 10.1089/ten.teb.2020.0373
Methodology: Kaixuan Li, Hanxiao Huang 9. Bielajew BJ, Donahue RP, Lamkin EK, Hu JC, Hascall VC,
Writing - original draft: Hanxiao Huang Athanasiou KA. Proteomic, mechanical, and biochemical
Writing- review & editing: Kaixuan Li characterization of cartilage development. Acta Biomater.
2022;143:52-62.
Ethics approval and consent to participate doi: 10.1016/j.actbio.2022.02.037
In this study, animal-related procedures were conducted 10. Sani M, Hosseinie R, Latifi M, et al. Engineered artificial
in accordance with the ethical standards approved by the articular cartilage made of decellularized extracellular
Volume 10 Issue 5 (2024) 289 doi: 10.36922/ijb.3371