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International Journal of Bioprinting 3D bioprinting for nanoparticle evaluation

Figure 9. Drug release model of drug-loaded nanoparticles (NPs). (A) Three-dimensional bioprinted model for evaluating the release and protective
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effects of minocycline (MH)-loaded NPs under oxidative stress conditions. (B) Controlled release of MH from MH-DS (dextran sulfate-based NPs
incorporated with MH) nanocomplexes in gelatin methacryloyl (GelMA).

with the aim of enhancing wound healing. This study the hydrophobic compound coumarin-6, achieving an
explored the synthesis, characterization, and biological encapsulation efficiency of approximately 70%.
evaluation of these NPs, focusing on their integration into The catechol-functionalized NPs demonstrated several
hydrogel bioinks for fabricating cell-laden 3D scaffolds. promising biological properties. In cell culture studies,
The primary objective is to utilize catechol-bearing NP2 and NP29 protected RAW 264.7 macrophages
polymeric NPs to improve wound healing. These NPs from oxidative stress induced by ROS and modulated
were synthesized using a nanoprecipitation method, and inflammatory responses. Additionally, they promoted the
two types of NPs with varied catechol content (2% and upregulation of vascular endothelial growth factor (VEGF)
29%, named NP2 and NP29) were created, exhibiting in fibroblasts and endothelial cells, crucial for angiogenesis
hydrodynamic diameters of 100 and 75 nm, respectively. and wound healing. These NPs were incorporated into a
These NPs were tested for their ability to encapsulate hydrogel bioink formulation composed of carboxymethyl

Volume 10 Issue 5 (2024) 22 doi: 10.36922/ijb.4273