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Matsugaki, et al.
analysis revealed the upregulated expression of Src flow was regulated accompanied with activated
in vertical flow stimuli, whereas the expression in Src expression . The obtained results indicate
[36]
static condition will be investigated in our future that osteocytes remodel dendritic cell processes to
study. Dendritic cell processes are anchored to the generate shear stress on their surface effectively
matrix wall through integrins, which experience under mechanical environmental stimuli. Our novel
fluid shear stress and transmit the mechanical bioprinting technique allowed the establishment of
stimuli into biochemical signals through multiple bone-mimetic anisotropic 3D structure involving
interactions with related proteins involving Src . the desired arrangement of osteocytes inside
[35]
The upregulated expression of Src in vertical group the matrix, which are fully functionalized and
means that the accelerated degradation of focal surrounded with bone-like niche, compared to the
adhesion located in crossing direction to fluid traditional monolayer 2D culture system (Figure 5).

Figure 5. Novel bone-mimetic anisotropic three-dimensional (3D) construct fabricated with bio-3D
printing technique. The obtained construct represents the 3D osteocytes networks which mimic the
living bone tissue. Osteocytes remodel their dendritic processes to generate shear stress on their surface,
resulting in the effective mechanosignaling.

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