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3D printing of anisotropic bone structure
           5 Conclusion                                        3.   Nakano T,  Kaibara  K, Tabata  Y,  et al., 2002, Unique
                                                                   Alignment  and  Texture  of Biological  Apatite  Crystallites
           A  3D bone-mimetic  anisotropic  microstructure         in Typical Calcified Tissues Analyzed by Microbeam x-ray
           was fabricated using the laminated layer method of      Diffractometer  System.  Bone, 31:479–87. DOI: 10.1016/
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           with  differentiation  into  mature  osteocytes.  The   Apatite (BAp) Crystallographic Orientation and Texture as a
           osteocytes embedded inside the scaffolds showed         New Index for Assessing the Microstructure and Function of
           an ordered arrangement of the cell body as well         Bone Regenerated by Tissue Engineering. Bone, 51:741–7.
           as dendritic cell process, which remodeled their        DOI: 10.1016/j.bone.2012.07.003.
           extrusion/degeneration  depending  on  the  flow    5.   Hennessy KM, Pollot BE, Clem WC, et al., 2009, The Effect
           direction. These findings indicate that functional      of Collagen I Mimetic Peptides on Mesenchymal Stem Cell
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                                                                   and Cell Compatibility of Alkali-free MgO-CaO-SrO-TiO2-
           Acknowledgments                                         P2O5 Glasses for Biomedical Applications. Biomed Glasses,
           This  research  was funded  by Grants-in-Aid  for       1:151–8. DOI: 10.1515/bglass-2015-0015.
           Scientific Research (S) (grant number 18H05254)     7.   Prewitz  MC, Seib  FP, von Bonin  M,  et  al.,  2013, Tightly
           and  Grants-in-Aid  for  Scientific  Research  (A)      Anchored  Tissue-mimetic  Matrices as Instructive Stem
           (grant number 20H00308).                                Cell Microenvironments.  Nat Methods, 10:788–794. DOI:
                                                                   10.1038/nmeth.2523.
           Conflicts of interest                               8.   Bonewald LF, 2011, The Amazing Osteocyte. J Bone Miner
                                                                   Res, 26:229–38. DOI: 10.1002/jbmr.320.
           The authors declare no conflicts of interest.       9.   Odagaki N, Ishihara Y, Wang Z, et al., 2018, Role of Osteocyte-
                                                                   PDL Crosstalk in Tooth Movement via SOST/Sclerostin. J
           Authors’ contributions
                                                                   Dent Res, 97:1374–82. DOI: 10.1177/0022034518771331.
           T. N. and A. MT. designed the study. T. M., A.      10.  Ganesh  T, Laughrey  LE, Niroobakhsh M,  et al., 2020,
           MK., and A. MT. carried out the experiments. T.         Multiscale Finite Element Modeling of Mechanical Strains
           M., A. MT., P. W., and T. N. contributed to the         and Fluid Flow in Osteocyte Lacunocanalicular  System.
           interpretation  of the results. A. MT. drafted the      Bone, 2020:115328. DOI: 10.1016/j.bone.2020.115328.
           manuscript, P. W. and T. N. revised the manuscript   11.  Ng WL, Chua CK, Shen YF, 2016, Print Me An Organ! Why
           content.  All the authors contributed  to the           We Are Not There Yet. Prog Polym Sci, 2016:101145.
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