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International Journal of Bioprinting 3D-Printed Zn/MgHA-PCL for angio/osteogenesis

Figure 6. Evaluation of the osteogenic ability of HA-PCLs, 10Zn@HA-PCLs, 5Mg10Zn@HA-PCLs, 10Mg10Zn@HA-PCLs, and 15Mg10Zn@HA-PCLs in
vitro. (A) Cells that have been in culture for 7 and 14 days using alkaline phosphatase (ALP) staining. (B) On day 21, cells were stained with alizarin red
S (ARS). (C) ALP activity of cells cultured for 7 days. (D) Quantification of cell mineralization on day 21. (E) The osteogenic-related gene expression of
each group of scaffolds (OPN, OCN, ALP, and RUNX2). *p < 0.05, **p < 0.01, ***p < 0.001; n = 4. Scale bars: 200 μm (A); 500 μm (B). Abbreviations: HA,
hydroxyapatite; PCL, polycaprolactone.

Volume 10 Issue 6 (2024) 294 doi: 10.36922/ijb.4243