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International Journal of Bioprinting Bioprinted plasma biocarriers for MSC delivery

Figure 2. Morphology and viability of bone marrow-derived mesenchymal stem cells (BMSCs) cultured in biocarriers for 96 h. (A) Z-stacks of live/dead-
type microscopy images, with cells counterstained with Hoechst 33342. (B) Total nuclei and dead cell count in random fields of live/dead microscopy
images are shown for the three hydrogels after 96 h of culture. (C) XTT-specific absorbance readings of BMSCs cultured in biocarriers at 24 and 96 h under
normal or pro-inflammatory (IL-1β) conditions. Scale bar: 500 µm. Abbreviations: FFP, fresh frozen plasma; PRP, platelet-rich plasma.

Table 1. Z-scores and p-values of non-inflamed and IL-1β-inflamed biocarriers obtained from Ingenuity Pathway Analysis Software
®
(IPA ) predictions for different relevant biological settings
Z-score (p-value)
Function Plasma-infused biocarriers
Non-inflamed IL-1β-inflamed
PRP-biocarrier 4.439 (3.58E-71) 3.854 (2.13E-69)
Cell survival
FFP-biocarrier 4.315 (3.58E-71) 3.556 (2.13E-69)
PRP-biocarrier 4.545 (7.50E-70) 4.108 (5.43E-68)
Cell viability
FFP-biocarrier 4.613 (7.50E-70) 3.821 (5.43E-68)
PRP-biocarrier −0.319 (9.02E-90) 0.091 (1.55E-89)
Necrosis
FFP-biocarrier −0.547 (9.02E-90) −0.893 (1.55E-89)
PRP-biocarrier −1.960 (5.37E-81) −1.979 (5.71E-81)
Apoptosis
FFP-biocarrier −2.814 (5.37E-81) −2.445 (5.71E-81)
Abbreviations: FFP, fresh frozen plasma; PRP, platelet-rich plasma.

Volume 10 Issue 6 (2024) 305 doi: 10.36922/ijb.4426