Page 315 - IJB-10-6

International Journal of Bioprinting Bioprinted plasma biocarriers for MSC delivery

Figure 4. Canonical pathways significantly activated by the secretome (Z-score > 2) of PRP- (A) and FFP-infused biocarriers (B). Abbreviations: FFP, fresh
frozen plasma; PRP, platelet-rich plasma.

3.4.1. Paracrine angiogenic activities of BMSCs under any type of plasma compared to those encapsulated solely
inflammatory conditions in GelMA (p < 0.001 for PRP biocarriers; p = 0.002 for FFP
®
Using IPA , we predicted and identified differences in biocarriers).
angiogenesis between groups. Table 2 presents Z-scores
for angiogenesis across all experimental conditions. A 3.4.2. Matrigel assays
Kruskal–Wallis test, followed by Bonferroni adjustment, To examine how inflammation, i.e., IL-1β exposure,
influenced angiogenesis, we performed Matrigel assays
revealed no significant differences in angiogenesis Z-scores
concerning platelet dose (p = 0.079). to examine the impact of the secretome released from
PRP biocarriers on the angiogenic potential of HUVECs
However, significant differences were noted in the after exposure to conditioned media collected under
angiogenic behavior of cells within scaffolds infused with inflammatory conditions (IL-1β, 50 ng/mL).

Volume 10 Issue 6 (2024) 307 doi: 10.36922/ijb.4426