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Zheng, et al.
A B C

Figure 8. (A) Scaffold implantation surgical procedure in rabbits. (B) Micro-computed tomography (Micro-CT) scan images and
quantification results of the defect area 12 weeks after the surgery; newly formed bone appeared as white dots on the CT images. (C) Microfil
angiography and micro-CT imaging results of the rabbits.

Conflicts of interest
The authors declare no conflicts of interest.

References

1. Trejo-Iriarte CG, Serrano-Bello J, Gutiérrez-Escalona R,
et al., 2019, Evaluation of Bone Regeneration in a Critical
Size Cortical Bone Defect in Rat Mandible Using MicroCT
and Histological Analysis. Arch Oral Biol, 101:165–71.
https://doi.org/10.1016/j.archoralbio.2019.01.010
2. Tarafder S, Davies NM, Bandyopadhyay A, et al., 2013,
3D Printed Tricalcium Phosphate Bone Tissue Engineering
Scaffolds: Effect of SrO and MgO Doping on In Vivo
Figure 9. Accumulative HA15 release profile of β-tricalcium Osteogenesis in a Rat Distal Femoral Defect Model. Biomater
phosphate/poly (Lactic-co-glycolic acid)/HA15 scaffold. Sci, 1:1250.
https://doi.org/10.1039/c3bm60132c
4. Conclusion 3. Giannoudis PV, Dinopoulos H, Tsiridis E, 2005, Bone
In this study, a β-TCP/PLGA bone tissue scaffold loaded Substitutes: An Update. Injury, 36:S20–7.
with osteogenesis-promoting drug HA15 was constructed https://doi.org/10.1016/j.injury.2005.07.029
by 3D printing technology. The scaffold loaded with the 4. Pape HC, Evans A, Kobbe P, 2010, Autologous Bone Graft:
HA15 delivery system has favorable biomechanical
properties comparable to those of the cancellous bone Properties and Techniques. J Orthop Trauma, 24:S36–40.
tissue and can promote cell differentiation into osteoblasts https://doi.org/10.1097/BOT.0b013e3181cec4a1
in vitro targeting HSPA5 and promote bone regeneration 5. Ebraheim NA, Elgafy H, Xu R, 2001, Bone-Graft Harvesting
in a rabbit bone defect model. Moreover, this scaffold can From Iliac and Fibular Donor Sites: Techniques and
enhance angiogenesis that has a significant role in more Complications. J Am Acad Orthop Surg, 9:210–8.
effective bone repair and regeneration. It was seen that https://doi.org/10.5435/00124635-200105000-00007
HA15 can target HSPA5 to inhibit ER stress and promote 6. Arrington ED, Smith WJ, Chambers HG, et al., 1996,
osteogenesis, along with the inhibition of ER stress,
reduced apoptosis, and induced autophagy in vivo. Overall, Complications of Iliac Crest Bone Graft Harvesting. Clin
this study might provide a theoretical approach for the Orthop Relat Res, 329:300–9.
treatment of bone defects with HA15-loaded biomaterials. https://doi.org/10.1097/00003086-199608000-00037

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