Page 138 - IJB-7-3

Multiresponsive Graphene-Oxide Embedded ECM Hydrogel for 3D Bioprinting
A B C

D E

F

Figure 5. SISMA-graphene oxide (GO) bioprinting. Adequate filament formation during extrusion aided
by a 3D bioprinter (A) and shape fidelity upon deposition (B) was observed for SISMA-GO composite
hydrogels, highlighting their suitability for bioprinting applications. (C) Flow sweep experiments of SISMA-
GO and SISMA hydrogels depict an appropriate shear-thinning behavior, with no observable difference in
the presence of GO. (D) Cell viability of human adipose tissue-derived mesenchymal stem cells (hAD-
MSC) 2 h, 3 days, and 7 days after bioprinting indicates high biocompatibility with SISMA-GO hydrogels
(n = 4). (E) Confocal microscopy images of hAD-MSCs embedded in bioprinted SISMA and SISMA-GO
constructs with stained nuclei in blue and stained F-actin filaments in green. Elongated morphologies are
observed after 6 days of incubation, indicating the presence of cell-matrix interactions that mediate cell
adhesion and spreading. (F) hAD-MSC proliferation in SISMA and SISMA-GO constructs, as measured
by resazurin reduction as a consequence of metabolic activity.

the formation of cell-matrix interactions that mediate favored hAD-MSCs proliferation, as evidenced by the
cell adhesion (i.e., cell-matrix adhesion complexes) . increased proliferation rate when compared to SISMA
[68]
Moreover, as observed in the magnified images of day constructs (Figure 5F). This is consistent with the
6 (Figure 5, bottom panel), hAD-MSCs in SISMA-GO previous reports where proteins adsorbed on GO surfaces
constructs exhibit a greater amount of filopodia than in have been shown to favor integrin-based cell adhesion by
SISMA constructs, indicating that the presence of GO providing anchoring sites that facilitate cell migration,
likely aids in cell adhesion. Notably, GO nanosheets also proliferation and function .
[69]

134 International Journal of Bioprinting (2021)–Volume 7, Issue 3