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Fabrication of Layered Gradient Brain-like Tissue by 3D Bioprinting
Figure 1. The integrated cell printing/culture equipment.
A B
Figure 2. Printing a layered gradient structure that mimics the cortex. (A) Schematic diagram of the layered gradient brain-like model\
designed in the current study. (B) The printing principle of the layered gradient brain-like structure.
Figure 3. Configuration process of the bio-ink.
The pH value of the bio-inks was adjusted to 7.4 U87 cells were mixed with the bio-inks to obtain the bio-
with 0.5M NaOH solution (Tianjin, Tianli), and then the inks with 1 × 10 cells/ml.
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74 International Journal of Bioprinting (2021)–Volume 7, Issue 3
