Page 99 - IJB-7-3
P. 99
Mao, et al.
supply. Finally, GFP-HUVECs and H9C2 cells were https://doi.org/10.1038/s41551-018-0224-z
mixed with inner-layer collagen and out-layer alginate 5. Miller JS, Stevens KR, Yang MT, et al., 2012, Rapid Casting
solution, respectively, to fabricate 3D pre-vascularized of Patterned Vascular Networks for Perfusable Engineered
cardiac constructs. It was noted that the HUVECs Three-dimensional Tissues. Nat Mater, 11:768.
encapsulated in collagen gel were able to spread and align https://doi.org/10.1038/NMAT3357
along the filaments surrounded by the H9C2 cells in the
3D constructs. These results demonstrate that this coaxial 6. Mao M, Bei HP, Lam CH, et al., 2020, Human-on-Leaf-Chip:
EHD bioprinting method has great potential to fabricate A Biomimetic Vascular System Integrated with Chamber-
complex 3D tissues or organs with vascular network. Specific Organs. Small, 16:2000546.
Acknowledgments https://doi.org/10.1002/smll.202000546
7. He J, Mao M, Liu Y, et al., 2013, Fabrication of Nature-
This work was financially supported by the National Inspired Microfluidic Network for Perfusable Tissue
Key Research and Development Program of China Constructs. Adv Healthc Mater, 2:1108–13.
(2018YFA0703000), the Key Research Project of Shaanxi https://doi.org/10.1002/adhm.201200404
Province (2020GXLH-Y-021), Guangdong Basic and
Applied Basic Research Foundation (2020B1515130002), 8. Lenoir L, Segonds F, Nguyen KA, et al., 2019, A Methodology
The Youth Innovation Team of Shaanxi Universities, to Develop a Vascular Geometry for In Vitro Cell Culture
and the Fundamental Research Funds for the Central Using Additive Manufacturing. Int J Bioprint, 5:238.
Universities. https://doi.org/10.18063/ijb.v5i2.238
Conflict of interest 9. Mirabella T, MacArthur JW, Cheng D, et al., 3D-printed
Vascular Networks Direct Therapeutic Angiogenesis in
No conflict of interest were reported by the authors. Ischaemia. Nat Biomed Eng, 1:0083.
Author contributions https://doi.org/10.1038/s41551-017-0083
10. Yao R, Alkhawtani AY, Chen R, et al., 2019, Rapid and
M.M. and H.L. designed the overall experimental plan Efficient In Vivo Angiogenesis Directed by Electro-assisted
and performed experiments. M.M. wrote the manuscript
with support from H.L. and J.H., and co-authors A.K., Bioprinting of Alginate/Collagen Microspheres with Human
Y.Z., L.W., and X.L. involved in the investigation and Umbilical Vein Endothelial Cell Coating Layer. Int J Bioprint,
analysis of results. J.H. and D.L. supervised the project 5:194.
and conceived the original idea. https://doi.org/10.18063/ijb.v5i2.1.194
11. Hasan A, Paul A, Vrana NE, et al., 2014, Microfluidic
References
Techniques for Development of 3D Vascularized Tissue.
1. Zhu W, Qu X, Zhu J, et al., 2017, Direct 3D Bioprinting Biomaterials, 35:7308–25.
of Prevascularized Tissue Constructs with Complex https://doi.org/10.1016/j.biomaterials.2014.04.091
Microarchitecture. Biomaterials, 124:106–15. 12. Ng WL, Chua CK, Shen YF, 2019, Print Me An Organ! Why
https://doi.org/10.1016/j.biomaterials.2017.01.042 We Are Not There Yet. Prog Polym Sci, 97:101145.
2. Baranski JD, Chaturvedi RR, Stevens KR, et al., 2013, https://doi.org/10.1016/j.progpolymsci.2019.101145
Geometric Control of Vascular Networks to Enhance 13. Li X, Liu B, Pei B, et al., 2020, Inkjet Bioprinting of
Engineered Tissue Integration and Function. Proc Natl Acad Biomaterials. Chem Rev, 120:10793–833.
Sci U S A, 110:7586–91. https://doi.org/10.1021/acs.chemrev.0c00008
https://doi.org/10.1073/pnas.1217796110 14. Jiang T, Munguia-Lopez JG, Flores-Torres S, et al., 2019,
3. Bertassoni LE, Cecconi M, Manoharan V, et al., 2014, Extrusion Bioprinting of Soft Materials: An Emerging
Hydrogel Bioprinted Microchannel Networks for Technique for Biological Model Fabrication. Appl Phys Rev,
Vascularization of Tissue Engineering Constructs. Lab Chip, 6:30.
14:2202–11. https://doi.org/10.1063/1.5059393
https://doi.org/10.1039/c4lc00030g 15. Ng WL, Lee JM, Zhou M, et al., 2020, Vat Polymerization-
4. Qiu Y, Ahn B, Sakurai Y, et al., 2018, Microvasculature- based Bioprinting-Process, Materials, Applications and
on-a-chip for the Long-term Study of Endothelial Barrier Regulatory Challenges. Biofabrication, 12:022001.
Dysfunction and Microvascular Obstruction in Disease. Nat https://doi.org/10.1088/1758-5090/ab6034
Biomed Eng, 2:453–63. 16. Kolesky DB, Homan KA, Skylar-Scott MA, et al., 2016,
International Journal of Bioprinting (2021)–Volume 7, Issue 3 95

