Page 99 - IJB-7-4
P. 99
Zhang, et al.
A
B
C
D
Figure 8. (A) Immunohistological staining for collagen type II of neocartilage at 3 months for the control group, pristine-bilayered construct
group, and GF-bilayered construct group (scale bar: 50 μm). (B) H & E staining and imaging of neo-bone at high magnification at 3 months
(blue arrows, trabecular structures; green arrows, typical osteoblasts; yellow arrows, osteocytes; red arrows, vascularization; scale bar:
100 μm). (C) Masson staining of neo-bone at 3 months (scale bar: 100 μm). (D) Immunohistological staining for OCN of neo-bone at
3 months (scale bar: 100 μm).
4. Conclusions Conflicts of interest
The present study has shown that each layer of scaffolds There are no conflicts of interest to declare.
had a suitable mechanical strength and degradation rate.
Furthermore, the scaffolds encapsulating TGF-β1 and Authors’ contributions
BMP-2 can act as a controlled released system and promote WM. F. planned the experiments; Q. Z. and Y. L.
directed differentiation of BMSCs. The in vivo experiments supervised the project; X. Z., ZX. L., R. X. and T. Y.
suggested that the scaffolds loaded with growth factors carried out the experiments; Y. L. and Q. Z. performed
promoted osteochondral regeneration in the rabbit knee joint the data analyses; X. Z. wrote the manuscript; QY. W.,
model. Altogether, the biomimetic bilayered scaffolds offer K. S., Y. Z., and K. Y. discussed the results, and reviewed
a novel option for application in osteochondral regeneration. and edited the manuscript.
Acknowledgments References
We would like to thank the Core Faility of Jiangsu 1. Lewis PB, McCarty LP 3 , Kang RW, et al., 2006, Basic
rd
Provincial People’s Hospital for its help in the detection
of experimental samples. Science and Treatment Options for Articular Cartilage
Injuries. J Orthop Sports Phys Ther, 36:717–27.
Funding 2. Kilian D, Ahlfeld T, Akkineni AR, et al., 2020, 3D Bioprinting
This work was supported by the National Natural Science of Osteochondral Tissue Substitutes in Vitro-Chondrogenesis
Foundation of China (No. 81974332). in Multi-layered Mineralized Constructs. Sci Rep, 10:8277.
International Journal of Bioprinting (2021)–Volume 7, Issue 4 95
