Page 129 - IJB-8-3

Zheng, et al.

A B

C

Figure 9. Release of silver ions by scaffold upregulated the level of ROS in bacteria. Non-cumulative (A) and cumulative (B) Ag
+
concentration released of Ag@PMoS /PGA scaffold. (C) Fluorescence microscope images of Escherichia coli incubated with
2
DCFH-DA after incubation with PGA, MoS /PGA, PMoS /PGA, and Ag@PMoS /PGA scaffolds with or without 808 nm NIR irradiation
2
2
2
(1 W/cm , 10 min).
2
change in ROS content in bacteria with or without NIR Acknowledgments
irradiation. While the Ag@PMoS /PGA scaffold can up-
2
regulate the ROS level in bacteria without NIR laser. This study was supported by the National Natural
And the ROS level in bacteria was more significantly Science Foundation of China (No. 51935014, 81871498,
upregulated when irradiated with NIR laser. 82072084, 81871494), Jiangxi Provincial Natural
Science Foundation of China (20192ACB20005),
4. Conclusion and Innovation for graduate students of Central South
University 2020 (2020zzts774, 2020zzts223). Special
A novel antitumor and antibacterial composite scaffold thanks to the technical platform and experiment
based on MoS NSs loaded with in situ grown Ag NPs was assistance of the Institute of Biological Additive
2
designed and constructed by SLS technology. This in situ Manufacturing of Jiangxi University of Science and
growth mode has a synergistic dispersion effect and helps Technology.
prevent the agglomeration of Ag NPs and MoS2 NSs in
the polymer scaffold. Meanwhile, MoS NSs can provide Conflict of interest
2
a barrier layer for Ag to react with external solution
+
and control its stable release. The data showed that the The authors declare that there are no competing interests.
composite scaffolds had stronger photothermal and Author contributions
antitumor effects and could induce tumor cell apoptosis.
The scaffolds also have stronger antibacterial function S.P. guided and supervised the project. L.Z. designed and
through upregulation of ROS in bacteria. In conclusion, supervised the experiments. L.Z., Y.Z., and T.H. carried
the porous Ag@PMoS /PGA composite scaffold may be out the experiments. L.Z. interpreted data and wrote the
2
a promising candidate for preventing tumor recurrence manuscript with support from Y.Z., T.H., and L.Y.; S.P.
and bacterial infection in reconstruction after bone tumor and L.Y. mentored the technical part of the project. All
surgery. authors read and approved the manuscript.

International Journal of Bioprinting (2022)–Volume 8, Issue 3 121

Please cite this article as: Zheng L, Zhong Y, He T, et al., 2022, A Codispersed Nanosystem of Silver-anchored MoS Enhances Antibacterial
2
and Antitumor Properties of Selective Laser Sintered Scaffolds, Int J Bioprint, 8(3):0025. http://doi.org/10.18063/ijb.v8i3.0025