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Zheng, et al.
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B C

Figure 7. The scaffolds selectively induced apoptosis in vitro. (A) FCM (flow cytometry) for apoptosis of MG63 cells after incubation with
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PGA, MoS /PGA, PMoS /PGA, and Ag@PMoS /PGA scaffolds with or without 808 nm NIR irradiation (1 W/cm , 10 min). (B) Statistics
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analysis of the apoptosis rate. (C) Western blot analysis of Bcl-2 and Bax protein expression in MG63 cells after incubation with PGA,
MoS /PGA, PMoS /PGA, and Ag@PMoS /PGA scaffolds with or without 808 nm NIR irradiation (1 W/cm , 10 min).
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induced by Ag@PMoS /PGA scaffolds after NIR death patterns include photothermal conversion efficiency
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irradiation was significantly increased by 2.7%, which and concentration of photothermal reagents, laser power
was consistent with the previous results. Bax and Bcl-2 density, and exposure time . Here, the data showed that
[55]
are Bcl-2 family genes. Bax promotes apoptosis and Bcl- the scaffolds MoS /PGA, PMoS /PGA, and Ag@PMoS /
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2 inhibits apoptosis. Western blot was performed to detect PGA induced the apoptosis of osteosarcoma cells under
the expression of Bax and Bcl-2 proteins in cells before NIR irradiation at low density (1 W/cm ) for 10 min.
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and after photothermal treatment. As shown in Figure 7C,
Bax protein was increased and Bcl-2 protein expression 3.7. Antibacterial function of scaffolds
was inhibited after photothermal treatment. Factors Clinically, it was well known that the bacterial infection
reported to influence photothermal therapy-induced cell was one problem causing orthopedic implant failure [56,57] .
International Journal of Bioprinting (2022)–Volume 8, Issue 3 119
Please cite this article as: Zheng L, Zhong Y, He T, et al., 2022, A Codispersed Nanosystem of Silver-anchored MoS Enhances Antibacterial
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and Antitumor Properties of Selective Laser Sintered Scaffolds, Int J Bioprint, 8(3):0025. http://doi.org/10.18063/ijb.v8i3.0025