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3D-bioprinted Ovary Initiated Puberty in the Model Mice
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Figure 8. Detection of the expression of ER-α, PR, inhibin-α and FSHR within the 3D scaffold encapsulating POCs and hydrogel encapsulating
POCs constructs after 4-week implantation. DAPI located in the nucleus (blue), the specific primary antibody located in the cell membrane
and cytoplasm (green). Green and blue superimposed to appear white, representing the expression of steroid hormone receptors in the groups
(white arrows). The expression of ER-α, Inhibin-α and FSHR were more in the 3D scaffold encapsulating POCs group than in the hydrogel
encapsulating POCs group. However, there was no significant difference in the expression of PR (P = 0.12). *P < 0.05.

278 International Journal of Bioprinting (2022)–Volume 8, Issue 3