Page 86 - IJB-8-3

Protein Nanoparticles Promote Cell Growth in 3D Bioprinted Constructs
process and positively influence cell viability suggest that Organs. Nat Biotechnol, 32:773–85.
this method can promote the survival of cells within 3D https://doi.org/10.1038/nbt.2958
printed constructs. Gas vesicles can be used as a bridge 7. Seol YJ, Kang HW, Lee SJ, et al., 2014, Bioprinting
to promote cell viability between construct formation and Technology and its Applications. Eur J Cardio Thorac Surg,
vascularization in the future.
46:342–8.
Acknowledgments https://doi.org/10.1093/ejcts/ezu148

The authors thank Dr. Thorsten Allers for generously 8. Li X, Liu B, Pei B, et al., 2020, Inkjet Bioprinting of
providing us with H. volcanii H1895 and its corresponding Biomaterials. Chem Rev, 120:10793–833.
vector pTA963. We thank the research intern, Alvin Huang, https://doi.org/10.1021/acs.chemrev.0c00008
for protein nanoparticle production and purification 9. Ng WL, Lee JM, Yeong WY, et al., 2017, Microvalve-based
assistance. The research reported in this publication was Bioprinting-process, Bio-inks and Applications. Biomater
supported by funding from King Abdullah University of Sci, 5:632–47.
Science and Technology and University of Jeddah.
10. Jentsch S, Nasehi R, Kuckelkorn C, et al., 2021, Multiscale
Conflict of interest 3D Bioprinting by Nozzle‐Free Acoustic Droplet Ejection.

The authors declare that they have no conflicts of interest. Small Methods, 5:2000971.
https://doi.org/10.1002/smtd.202000971
Author contributions 11. Dou C, Perez V, Qu J, et al., 2021, A State‐of‐the‐Art Review

C.A.E.H. and M.R. conceived, directed the study and of Laser‐Assisted Bioprinting and its Future Research Trends.
planned the experiments. R.K. designed and constructed ChemBioEng Rev, 8:517–34.
the GVNP expression system and performed the GVNP https://doi.org/10.1002/cben.202000037
characterization with D.R. and S.M. S.A. and S.G. 12. Fu Z, Naghieh S, Xu C, et al., 2021, Printability in Extrusion
performed the biocompatibility study. Z.K and K.K Bioprinting. Biofabrication, 13:033001.
designed the 3D printing system and performed the https://doi.org/10.1088/1758-5090/abe7ab
bioprinting. S.A. carried out the SEM and confocal
imaging. S.A. prepared the manuscript with assistance 13. Ng WL, Lee JM, Zhou M, et al., 2020, Vat Polymerization-
from R.K. and S.G. All authors provided input on the based Bioprinting-process, Materials, Applications and
study and experimental design, analysis, and manuscript. Regulatory Challenges. Biofabrication, 12:022001.
https://doi.org/10.1088/1758-5090/ab6034
References 14. Ravnic DJ, Leberfinger AN, Koduru SV, et al., 2017,

1. Cui H, Nowicki M, Fisher JP, et al., 2017, 3D Bioprinting for Transplantation of Bioprinted Tissues and Organs: Technical and
Organ Regeneration. Adv Healthc Mater, 6:1601118. Clinical Challenges and Future Perspectives. Ann Surg, 266:48-58.
https://doi.org/10.1002/adhm.201601118 https://doi.org/10.1097/sla.0000000000002141
2. Khademhosseini A, Langer R, 2016, A decade of Progress in 15. Hauser CA, Zhang SG, 2010, Designer Self-assembling
Tissue Engineering. Nat Protoc, 11:1775–81. Peptide Nanofiber Biological Materials. Chem Soc Rev,
https://doi.org/10.1038/nprot.2016.123 39:2780–90.
3. Matai I, Kaur G, Seyedsalehi A, et al., 2020, Progress in https://doi.org/10.1039/b921448h
3D Bioprinting Technology for Tissue/Organ Regenerative 16. Chen C, Bang S, Cho Y, et al., 2016, Research Trends in
Engineering. Biomaterials, 226:119536. Biomimetic Medical Materials for Tissue Engineering: 3D
https://doi.org/10.1016/j.biomaterials.2019.119536 Bioprinting, Surface Modification, Nano/Micro-technology
4. Ng WL, Chua CK, Shen YF, 2019, Print me an Organ! Why and Clinical Aspects in Tissue Engineering of Cartilage and
we are not there yet. Prog Polym Sci, 97:101145. Bone. Biomater Res, 20:10.
https://doi.org/10.1016/j.progpolymsci.2019.101145 https://doi.org/10.1186/s40824-016-0057-3
5. Bishop ES, Mostafa S, Pakvasa M, et al., 2017, 3-D 17. Visconti RP, Kasyanov V, Gentile C, et al., 2010, Towards
Bioprinting Technologies in Tissue Engineering and Organ Printing: Engineering an Intra-organ Branched
Regenerative Medicine: Current and Future Trends. Genes Vascular Tree. Expert Opin Biol Ther, 10:409–20.
Dis, 4:185–95. https://doi.org/10.1517/14712590903563352
https://doi.org/10.1016/j.gendis.2017.10.002 18. Kolesky DB, Homan KA, Skylar-Scott MA, et al., 2016,
6. Murphy SV, Atala A, 2014, 3D Bioprinting of Tissues and Three-dimensional Bioprinting of Thick Vascularized

78 International Journal of Bioprinting (2022)–Volume 8, Issue 3