Page 55 - IJB-9-1

International Journal of Bioprinting

RESEARCH ARTICLE
In situ defect detection and feedback control

with three-dimensional extrusion-based
bioprinter-associated optical coherence

tomography

1
1,2
1
Shanshan Yang *, Qi Chen , Ling Wang *, Mingen Xu *
1,2
1 School of Automation, Hangzhou Dianzi University, Hangzhou, China
2 Key Laboratory of Medical Information and 3D Bioprinting, Zhejiang Province, Hangzhou, China

Abstract

Extrusion-based three-dimensional (3D) bioprinting is one of the most common
methods used for tissue fabrication and is the most widely used additive
manufacturing technique in all industries. In extrusion-based bioprinting, printing
defects related to material deposition errors lead to a significant deviation from
shape to function between the printed construct and design model. Using 3D
extrusion-based bioprinter-associated optical coherence tomography (3D P-OCT),
*Corresponding authors: an in situ defect detection and feedback system was presented based on the accurate
Shanshan Yang defect analysis and location, and a pre-built feedback mechanism. Using 3D P-OCT,
(yangshan@hdu.edu.cn)
Ling Wang multi-parameter quantification of the material deposition was carried out in real
(lingw@hdu.edu.cn) time, including the filament size, layer thickness, and layer fidelity. The material
Mingen Xu deposition errors under different paths were quantified and located specifically,
(xumingen@hdu.edu.cn) including the start-stop points, straight-line path, and turnarounds. The pre-built
Citation: Yang S, Chen Q, feedback mechanism involving the control inputs, such as printing path, pressure,
Wang L, et al., 2023, In situ defect and velocity, provided the basis for in situ defect detection and real-time feedback
detection and feedback control
with three-dimensional extrusion- control. In particular, the second printing repair can be performed after the broken
based bioprinter-associated optical filament defect is detected and located. After printing, fidelity can be quantitatively
coherence tomography. Int J analyzed based on the point cloud registration between the 3D P-OCT result and the
Bioprint, 9(1): 624. design model. In conclusion, 3D P-OCT enables in situ defect detection and feedback
http://doi.org/10.18063/ijb.v9i1.624
control, broken filament repair, and 3D fidelity analysis to achieve high-fidelity
Received: June 7, 2022 printing from shape to function.
Accepted: July 14, 2022
Published Online: October 27, 2022 Keywords: Optical coherence tomography; Extrusion-based bioprinting; Process
Copyright: © 2022 Author(s). monitoring; Defect detection; Feedback control; High fidelity
This is an Open Access article
distributed under the terms of the
Creative Commons Attribution
License, permitting distribution,
and reproduction in any medium, 1. Introduction
provided the original work is
properly cited. With its potential to fabricate three-dimensional (3D) biomimetic functional
tissue constructs and organs, 3D bioprinting has been applied in organ printing [1,2] ,
Publisher’s Note: Whioce
[3]
[4]
Publishing remains neutral with microvasculature printing , disease modeling , and scaffold fabrication for tissue
regard to jurisdictional claims in regeneration [5,6] . According to different prototyping principles and printed materials,
published maps and institutional
affiliations. 3D bioprinting follows three main approaches: droplet-based, extrusion-based,

Volume 9 Issue 1 (2023) 47 https://doi.org/10.18063/ijb.v9i1.624