Page 52 - IJB-9-1

International Journal of Bioprinting Effect of ionic crosslinking on composite membranes

The elongation at break of the cross-linked alginate-based Funding
composite bioscaffolds showed almost the similar values in
a range of 5.0 – 5.2%. None.

Introduction of decellularized extracellular matrix derived Conflict of interest
from porcine tissue into the materials showed relatively The authors declare no potential conflicts of interest with
good mechanical properties and good thermal stability, respect to the research, authorship, and/or publication of
which could be employed for biomedical applications using this article.
bioprinting technology and designing bioinks containing
ALG and SFDDS with ALG/SFDDS in the ratio of 90/10 Author contributions
– 80/20. Furthermore, the cells might be introduced in Supervision: Ching-Cheng Huang
the bioinks for tissue engineering applications. Naghieh Conceptualization: Ching-Cheng Huang
et al. reported that the mechanical behavior of 3D-printed Investigation: Jun Xu
alginate scaffolds and the important printing parameters, Formal analysis: Jun Xu
such as printing speed, printing pressure, or material inflow, Writing – original draft: Run-Miao Yang
were the essential information in extrusion bioprinting Writing – review & editing: Ching-Cheng Huang
for the preparation of 3D-printed macroscaffolds . In
[29]
this study, the 3D-printed scaffolds containing micro- Ethics approval and consent to participate
bioscaffolds were considered and designed. The supercritical
fluids-decellularization technique was applied to obtain Not applicable.
decellularized dermal-based micro-bioscaffolds. The Consent for publication
designed 3D-printed scaffolds containing supercritical
fluids-decellularized dermal-based micro-bioscaffolds were Not applicable.
successfully prepared by extrusion bioprinting and freeze- Availability of data
drying. For specific medical applications of 3D-printed
macro-scaffolds containing the resulting micro-bioscaffolds The original contributions presented in the study are
in the future, the printing parameters for the preparation included in the article, and further inquiries can be directed
of 3D-printed macro-scaffolds might be useful to obtain to the corresponding author.
preferable 3D-printed scaffolds .
[29]
References
4. Conclusions 1. Huang CC, Chen YJ, Liu HW, 2021, Characterization
A new cross-linked alginate-based composite bioscaffold of composite nano-bioscaffolds based on collagen
containing supercritical fluids-decellularized dermal-based and supercritical fluids-assisted decellularized fibrous
bioscaffolds, which could maintain the microstructure of extracellular matrix. Polymers, 13: 4326.
integrity extracellular matrix, was successfully prepared. https://doi.org/10.3390/polym13244326
Effect of Ca ion penetration on structural stability and 2. Tappa K, Jammalamadaka U, 2018, Novel biomaterials used
2+
thermal stability of the resulting cross-linked alginate- in medical 3d printing techniques. J Funct Biomater, 9: 17.
based composite bioscaffold was studied with different https://doi.org/10.3390/jfb9010017
ionic crosslinking. Furthermore, several types of
microstructures were proposed within the cross-linked 3. Rhee S, Puetzer JL, Mason BN, et al., 2016, 3D bioprinting
alginate-based composite bioscaffolds in evidence of DTG of spatially heterogeneous collagen constructs for cartilage
and morphology results. The cross-linked alginate-based tissue engineering. ACS Biomater Sci Eng, 2: 1800–1805.
composite bioscaffolds exhibited good thermal stability, https://doi.org/10.1021/acsbiomaterials.6b00288
and its T dmax could reach up to 400°C. Furthermore, the 4. Laronda MM, Rutz AL, Xiao S, et al., 2017, A bioprosthetic
cross-linked alginate-based composite bioscaffolds showed ovary created using 3D printed microporous scaffolds
a relatively good structural stability, indicating that it could restores ovarian function in sterilized mice. Nat Commun, 8:
be a potential biomaterial for bioprinting applications. 15261.
https://doi.org/10.1038/ncomms15261
Acknowledgments
5. Markstedt K, Mantas A, Tournier I, et al., 2015, 3D
Authors would like to acknowledge the Taiwan PARSD bioprinting human chondrocytes with nanocellulose
Pharmaceutical Technology Consultants Ltd. Company alginate bioink for cartilage tissue engineering applications.
for technical support. Biomacromolecules, 16: 1489–1496.

Volume 9 Issue 1 (2023) 44 http://doi.org/10.18063/ijb.v9i1.625