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International Journal of Bioprinting Enhanced osteogenesis in gelatin releasing bioink

bone tissue-targeted bioink. After 3 weeks in cell culture Ethics approval and consent to participate
media, it exhibited the ability to enhance the osteogenic Not applicable.
differentiation behavior of encapsulated cells.

4. Conclusions Consent for publication

In this study, MA-alginate and gelatin were blended to Not applicable.
prepare multi-component hydrogel samples. Compared Availability of data
to the single-component MA-alginate hydrogel, the
two-component hydrogels showed increased swelling Not applicable.
properties because the released gelatin left empty spaces in
the hydrogels. Also, the two-component hydrogel samples References
induced greater cell viability and exhibited the potential to
induce osteogenic behavior in external and encapsulated 1. Santoni S, Gugliandolo SG, Sponchioni M, et al., 2022,
cells due to the effects of released and remaining 3D bioprinting: Current status and trends—A guide to
gelatin. The optimal printing conditions for these multi- the literature and industrial practice. Bio-Design Manuf,
component hydrogels were investigated and determined, 5(1):14–42.
and these multi-component hydrogels were found to have https://doi.org/10.1007/s42242-021-00165-0
improved printability with higher fidelity than single- 2. Ahadian S, Khademhosseini A, 2018, A perspective on
component hydrogel, specifically MA-alginate. Therefore, 3D bioprinting in tissue regeneration. Bio-Design Manuf,
we anticipate that the gelatin-containing alginate-based 1(3):157–160.
bioink developed in this study might be utilized to induce https://doi.org/10.1007/s42242-018-0020-3
osteogenesis within and external to the hydrogel in bone
tissue regeneration. 3. Groll J, Burdick JA, Cho DW, et al., 2018, A definition
of bioinks and their distinction from biomaterial inks.
Acknowledgments Biofabrication, 11(1):013001.
https://doi.org/10.1088/1758-5090/aaec52
The schematic image in Figure 1 was created with
BioRender.com. 4. Bian L, 2020, Functional hydrogel bioink, a key challenge of
3D cellular bioprinting. APL Bioeng, 4(3):030401–030401.
Funding https://doi.org/10.1063/5.0018548

This study was supported by the Fundamental Research 5. Cui X, Li J, Hartanto Y, et al., 2020, Advances in extrusion
Program of the Korea Institute of Material Science 3D bioprinting: A focus on multicomponent hydrogel-based
(PNK8170) and the Industrial Strategic Technology bioinks. Adv Healthc Mater, 9(15):1901648.
Development Program (20015148) funded by the Ministry https://doi.org/10.1002/adhm.201901648
of Trade, Industry & Energy (MOTIE, Korea). 6. Bell JH, Haycock JW, 2012, Next generation nerve guides:
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Conflict of interest Tissue Eng Part B Rev, 18(2):116–128.
The authors declare that the research was conducted in the https://doi.org/10.1089/ten.TEB.2011.0498
absence of any commercial or financial relationships that 7. Kyle S, Jessop ZM, Al-Sabah A, et al., 2017, ‘Printability’
could be construed as a potential conflict of interest.
of candidate biomaterials for extrusion based 3D printing:
State-of-the-art. Adv Healthc Mater, 6(16):1700264.
Author contributions
https://doi.org/10.1002/adhm.201700264
Conceptualization: Jueun Kim, Honghyun Park, Hui-Suk 8. Sellaro TL, Ranade A, Faulk DM, et al., 2010, Maintenance
Yun of human hepatocyte function in vitro by liver-derived
Methodology: Jueun Kim, Honghyun Park, Yeong-Jin Choi, extracellular matrix gels. Tissue Eng Part A, 16(3):1075–
Hui-Suk Yun 1082.
Project administration: Honghyun Park, Hui-Suk Yun https://doi.org/10.1089/ten.tea.2008.0587
Supervision: Honghyun Park, Hui-Suk Yun
Validation: Jueun Kim 9. Pati F, Jang J, Ha D-H, et al., 2014, Printing three-dimensional
Writing – original draft: Jueun Kim tissue analogues with decellularized extracellular matrix
Writing – review & editing: Chang-Woo Gal, Aram Sung, bioink. Nat Commun, 5(1):3935.
Yeong-Jin Choi, Honghyun Park, Hui-Suk Yun https://doi.org/10.1038/ncomms4935

Volume 9 Issue 2 (2023) 155 https://doi.org/10.18063/ijb.v9i2.660