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International Journal of Bioprinting Enhanced osteogenesis in gelatin releasing bioink

Figure 8. Printability test by (a) pressure/nozzle and (b) strut width and live/dead assay on fabricated scaffold: (c) 7:3 MA-alginate/gelatin and (d) 5:5
MA-alginate/gelatin.

have confirmed via a gelatin release test that this multi- higher ALP activity when exposed to gelatin-containing
component hydrogel can be released under cell culturing hydrogels than when exposed to gelatin-free hydrogels.
conditions. We also found that some gelatin remained in It was also clear from the gene expression results that the
the MA-alginate hydrogel via fluorescence imaging. gelatin released from the hydrogels had a positive effect on

To investigate the effects of the released and retained osteogenic differentiation. When the effects of the retained
gelatin on cells external to and encapsulated within the gelatin on encapsulated cells were studied (via ALP
hydrogel, the DNA levels, ALP activity, and gene expression activity and gene expression analysis), it was found that the
of the cells were analyzed. External cells were found to have hydrogels with more retained gelatin had higher potential

Volume 9 Issue 2 (2023) 153 https://doi.org/10.18063/ijb.v9i2.660