Page 52 - IJB-9-4

International Journal of Bioprinting 3D-Bioprinted human lipoaspirate-derived cell-laden skin constructs

after implantation. A specific microenvironment that Ethics approval and consent to participate
provides complex communication is critical in regulating The use of clinical lipoaspirates was approved by the Ethics
cell survival and differentiation . Previous studies have Committee of the PLA General Hospital (No. 2022KY135-
[65]
confirmed that ADSCs can differentiate in vivo and adapt KS001). All animal experiments were approved by the ethics
to microenvironmental conditions . In skin regeneration committee of the Fourth Medical Center of PLA General
[66]
studies, MSCs have been shown to differentiate into Hospital (No. 2022-X18-03). Informed consent to participate
keratinocytes, dermal fibroblasts, and other skin in this study was obtained from the human subjects.
components [67-70] . Therefore, our subsequent studies will
focus on the possible cellular fates of ADSCs and the Consent for publication
underlying regulatory mechanisms after implantation of
the 3D-printed scaffolds. Written informed consent has been obtained from the
human subjects involved in this study for publishing their
5. Conclusion data in the current paper.
The ADSC-laden 3D-printed scaffolds fabricated herein Availability of data
could address several clinical needs for treating major
skin defects, such as sufficient material source, timely All data that support the findings of this study are included
preparation, and efficacy. It is important to emphasize within the article.
the rapid timeline for the preparation of these scaffolds,
allowing sufficient material to be quickly obtained from References
human lipoaspirate for the fabrication of the clinically
needed scaffolds. We found that the 3D-printed ADSC- 1. Fu X, 2020, Wound healing center establishment and new
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laden adECM–GelMA–HAMA scaffold can accelerate quality in China. Burns Trauma, 8:tkaa038.
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may consequently become a new treatment method for 2. Nussbaum SR, Carter MJ, Fife CE, et al., 2018, An economic
full-thickness skin defects. evaluation of the impact, cost, and Medicare policy
implications of chronic nonhealing wounds. Value Health,
Acknowledgments 21(1):27–32.
https://doi.org/10.1016/j.jval.2017.07.007
We thank the technical assistance of Dr. Jiachen Sun from
the Department of Dermatology, Peking University Third 3. Wurzer P, Keil H, Branski LK, et al., 2016, The use of skin
substitutes and burn care—A survey. J Surg Res, 201(2):
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Funding https://doi.org/10.1016/j.jss.2015.10.048
4. Riha SM, Maarof M, Fauzi MB, 2021, Synergistic effect
No fundings were received for this study. of biomaterial and stem cell for skin tissue engineering
in cutaneous wound healing: A concise review. Polymers
Conflict of interest (Basel), 13(10):1546.
The authors declare no conflict of interest. https://doi.org/10.3390/polym13101546
5. Stone RC, Stojadinovic O, Rosa AM, et al., 2017, A
Author contributions bioengineered living cell construct activates an acute wound
healing response in venous leg ulcers. Sci Transl Med,
Conceptualization: Minliang Chen, Dequan Zhang 9(371):eaaf8611.
Data curation: Dequan Zhang, Huijuan Fu, Jinshi Zeng https://doi.org/10.1126/scitranslmed.aaf8611
Formal analysis: Dequan Zhang
Investigation: Dequan Zhang, Qiang Fu, Jinshi Zeng 6. Eudy M, Eudy CL, Roy S, 2021, Apligraf as an alternative
Methodology: Dequan Zhang to skin grafting in the pediatric population. Cureus,
13(7):e16226.
Resources: Qiang Fu
Software: Litao Jia https://doi.org/10.7759/cureus.16226
Supervision: Minliang Chen 7. Dai C, Shih S, Khachemoune A, 2020, Skin substitutes for
Validation: Dequan Zhang, Huijuan Fu acute and chronic wound healing: An updated review. J
Writing – original draft: Dequan Zhang, Qiang Fu Dermatol Treat, 31(6):639–648.
Writing – review & editing: Dequan Zhang, Minliang Chen https://doi.org/10.1080/09546634.2018.1530443

Volume 9 Issue 4 (2023) 44 https://doi.org/10.18063/ijb.718