Page 120 - IJB-9-5

International Journal of Bioprinting

RESEARCH ARTICLE
DLP-printed GelMA-PMAA scaffold for bone

regeneration through endochondral ossification

Jianpeng Gao 1,2† , Hufei Wang 3,4† , Ming Li , Zhongyang Liu , Junyao Cheng ,
1†
1
1, 2
Xiao Liu , Jianheng Liu *, Xing Wang *, Licheng Zhang *
1
3, 4
1, 2
1
1 Department of Orthopaedics, Chinese PLA General Hospital, 100039 Beijing, China
2 Medical School of Chinese PLA, 100039 Beijing, China
3 Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of
Sciences, 100190 Beijing, China
4 University of Chinese Academy of Sciences, 100049 Beijing, China
(This article belongs to the Special Issue: Additive Manufacturing of Functional Biomaterials)
Abstract

Intramembranous ossification (IMO) and endochondral ossification (ECO) are
two pathways of bone regeneration. The regeneration of most bone, such as limb
bone, trunk bone, and skull base bone, mainly occurs in the form of endochondral
ossification, which has also become one of the effective ways for bone tissue
engineering. In this work, we prepared a well-structured and biocompatible
† These authors contributed equally methacrylated gelatin/polymethacrylic acid (GelMA/PMAA) hydrogel by digital
to this work.
light processing (DLP) printing technology, which could effectively chelate iron ions
*Corresponding authors: Jianheng and continuously activate the hypoxia-inducible factor-1 alpha (HIF-1α) signaling
Liu pathway to promote the process of endochondral ossification and angiogenesis. The
(jianhengliu@126.com)
Xing Wang incorporation of PMAA endowed the hydrogel with remarkable viscoelasticity and
(wangxing@iccas.ac.cn) high efficacy in chelation of iron ions, giving rise to the activation of HIF-1α signaling
Licheng Zhang pathway, improving chondrogenic differentiation in the early stage, and facilitating
(zhanglcheng301@163.com) vascularization in the later stage and bone remodeling. Therefore, the findings have
Citation: Gao J, Wang H, Li M, significant implications on DLP printing technology of endochondral osteogenesis
et al., 2023, DLP-printed induced by the iron-chelating property of biological scaffold, which will provide an
GelMA-PMAA scaffold for bone effective way in the development of novel bone regeneration.
regeneration through endochondral
ossification. Int J Bioprint, 9(5): 754.
https://doi.org/10.18063/ijb.754
Keywords: Endochondral ossification; Digital light processing; Hydrogel;
Received: February 1, 2023 Bone tissue engineering
Accepted: March 31, 2023
Published Online: May 16, 2023
Copyright: © 2023 Author(s).
This is an Open Access article 1. Introduction
distributed under the terms of the
Creative Commons Attribution Bone defects caused by various factors such as aging, disease, and trauma are extremely
License, permitting distribution,
and reproduction in any medium, harmful to the body; therefore, effective treatments are necessary to achieve bone tissue
provided the original work is repair and regeneration [1, 2] . Intramembranous ossification (IMO) and endochondral
properly cited. ossification (ECO) are two forms of bone regeneration [3-6] . At the bone formation site,
Publisher’s Note: Whioce vascular hyperplasia leads to an adequate supply of nutrients and oxygen, and the
Publishing remains neutral with mesenchymal cells can differentiate into osteoblasts, which secrete osteoids and calcify
regard to jurisdictional claims in
published maps and institutional into the bone matrix to form bone tissue [7, 8] . However, intramembranous osteogenesis
affiliations. often results in failure of bone regeneration due to insufficient early angiogenesis [9-11] .

Volume 9 Issue 5 (2023) 112 https://doi.org/10.18063/ijb.754