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International Journal of Bioprinting        Guide about the effects of sterilization on 3D-printed materials for medicine



            Table 8. Mechanical properties of the 3D-printed Durable
             Parameters                                     Mechanical properties
                           Tensile strength (MPa)  Young’s modulus (MPa)  Elongation at yield (%)  Elongation at break (%)
             Control       18.46 ± 0.36        673 ± 37             15.39 ± 13.27        23.92 ± 0.60
             HPO           17.83 ± 0.24        665 ± 54             16.27 ± 14.02        20.16 ± 7.47
             AU121         17.67 ± 0.55        685 ± 65             15.98 ± 13.92        25.38 ± 2.86
             AU134         15.73 ± 0.09        659 ± 36             20.83 ± 5.43         21.15 ± 5.37
            Data are represented as mean ± SD. N = 3 Durable Control/group; N = 3 Durable HPO/group; N = 3 Durable AU121/group; N = 3 Durable AU134/group.
































            Figure 9. PA12 sterilization comparison. Data are represented as mean values. N = 3 PA12 Control/group; N = 3 PA12 HPO/group; N = 3 PA12 AU121/
            group; N = 3 PA12 AU134/group.

            3.1.4. VERO                                        HPO sterilization imparted a very small effect on the tensile
            Figure 6 shows the mechanics of the control sample and   strength of this material, with a difference of 0.64% as
            3D-printed VERO samples sterilized by HPO, AU121, and   compared to the control group. The AU121 and the AU134
            AU134. Both AU121 and AU134 slightly decreased the   methods resulted in a tensile strength difference of 14%
            mechanical properties of the tested VERO samples, which   and 36.04%, respectively. Interestingly, tensile strength was
            had a tensile strength of 29.16 ± 1.49 MPa and 30.26 ± 3.02   increased in the case of AU121 sterilization.
            MPa respectively, as compared to the 31.32 ± 2.25 MPa of
            control samples tested. The HPO method increased the   3.1.6. Durable
            tensile strength by 15% (36.07 ± 4.73 MPa). There were no   Figure 8 shows the mechanics of the control sample and
            significant differences in tensile strength impact between   3D-printed Durable samples sterilized by HPO, AU121,
            AU121 and AU134; however, AU134  showed a bigger   and AU134. Table 8 shows different mechanical properties
            impact. Table 6 shows different mechanical properties of   of Durable material with different methods. It was found
            VERO with different methods.                       that a higher temperature to which the sample is exposed
                                                               caused a bigger drop in the tensile strength. HPO resulted
            3.1.5. Surgical Guide resin                        in a tensile strength difference of 3.53%, as compared to
            Figure 7 shows the mechanics of the control sample and   the tensile strength of control sample. The AU121 method
            3D-printed Surgical Guide resin samples sterilized by HPO,   resulted in a tensile strength difference of 4.47%, which is
            AU121,  and  AU134.  Table  7  shows  different  mechanical   quite close to that of the HPO method. The impact of heat
            properties of Surgical Guide resin with different methods.   was noticed in AU134 method with a bigger difference in


            Volume 9 Issue 5 (2023)                        155                         https://doi.org/10.18063/ijb.756
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