Page 379 - IJB-9-5

International Journal of Bioprinting Vascularized bone regeneration

Scheme 1. (A) Schematic diagram of the synthesis process, crosslinking conditions, and principle of photocured PMBG. (B) Flowchart for the preparation
of PMBG/TCP bone repair scaffold. (C) High biologically active PMBG/TCP scaffold used for bone defect repair.

MO, USA), as well as tetraethyl orthosilicate (TEOS), subsequently suspended under vacuum at 40°C to yield
triethyl phosphate (TEP), hydrochloric acid (HCl), PMBG gels.
3-(trimethoxysilyl) propyl methacrylate (TMSPMA),
and glutaraldehyde from Aladdin Reagent (Shanghai) 2.3. Preparation of 3D-printed scaffold
Co. (Shanghai, China). We obtained ethanol and Chemical precipitation was used to obtain TCP powder.
Ca(NO ) ·4H O from Sinopharm Chemical Reagent Co., First, Ca(NO ) ·4H O and (NH ) HPO were separately
4 2
4
2
3 2
2
3 2
Ltd. (Shanghai, China). dissolved in ultrapure water. The solutions were
continuously stirred, while the pH was maintained between
7.0 and 7.5 by adding the Ca(NO ) solution dropwise. After
2.2. Surface functionalization of bioactive glass completion of the reaction, the product was centrifuged
3 2
Photocurable MBG (PMBG) was synthesized using and washed four times with ultrapure water and once with
3-(trimethoxysilyl) propyl methacrylate as the modifier. ethanol to separate and purify the precipitate.
The synthesis process involved mixing F-127, TEOS,
Ca(NO ) ·4H O, HCl (0.5 M), 3-(trimethoxysilyl) To fabricate the PMBG/TCP composite scaffold, 3D
2
3 2
propyl methacrylate, and TEP with 360 g of ethanol. The printing (using Biobuild-S, China) was employed. PMBG
mixture was incubated for 24 h at 37°C with stirring and gels were mixed with TCP powder and LAP (at 0.5 wt%),
Volume 9 Issue 5 (2023) 371 https://doi.org/10.18063/ijb.767