Page 382 - IJB-9-5

International Journal of Bioprinting Vascularized bone regeneration

Figure 1. Ultrafast photocured mesoporous bioglasses synthesis and characterization. (A, B) The photo-crosslinking properties of PMBG. © FTIR spectra
1
of MBG as well as PMBG. (D) H NMR spectroscopy of MBG as well as PMBG. (E–H) PMBG, PMBG/5TCP (5% TCP), PMBG/10TCP (10% TCP), and
PMBG/20TCP (20% TCP) gels photosensitive rheology. (I) Thermogravimetric analysis of PMBG scaffolds and PMBG/5TCP scaffolds, PMBG/10TCP
scaffolds, and PMBG/20TCP scaffolds.

temperature. The sections were examined under LSCM at 1058 cm in the FTIR spectra. This modification was
-1
after counterstaining the nuclei with DAPI. further verified using H NMR spectra. PMBG exhibited
1
new signals at 5.58, 6.15, and 1.93 ppm, which matched
2.15. Statistical analysis the protons of the double bond of TMSPMA. This suggests
The mean and standard deviation of each measurement that TMSPMA has been successfully incorporated into
were computed using descriptive statistics. To assess group the molecules hydrolyzed or condensed from TEOS.
differences, one-way analysis of variance (ANOVA) and The time-dependent rheological properties of PMBG,
Tukey’s post hoc test were used. For statistical research, PMBG/5TCP (5% TCP), PMBG/10TCP (10% TCP),
GraphPad Prism software (GraphPad Software Inc., USA) and PMBG/20TCP (20% TCP) were evaluated under 5%
was used. A result was deemed statistically different if *p < strain and 25°C using rheological tests (Figure 1E–H).
0.05, **p < 0.01, and *** p < 0.001. Before blue light irradiation, a loss modulus (G″) value

3. Results and discussion greater than the storage modulus (G′) value was observed,
indicating that each group was a viscous fluid. When
3.1. PMBG gel synthesis and characterization blue light was irradiated, G′ and G″ sharply increased,
The precursor of PMBG was synthesized by partially then immediately formed a crossover point, and rapidly
replacing tetraethyl orthosilicate (TEOS) with reached a plateau, indicating that both groups underwent a
3-(trimethoxysilyl) propyl methacrylate (TMSPMA). light-triggered instantaneous sol-gel transition, ultimately
PMBG exhibited rapid light-induced crosslinking achieving rapid and complete gelation and forming stable
properties and could be cured within 5 s (Figure 1A and B). gels (Videoclip S1 in Supplementary File). These results
FTIR spectra (Figure 1C) showed a peak at 1718.2 cm indicate that the prepared PMBG and inks doped with
-1
for PMBG, which was absent in MBG. In addition, both various proportions of TCP particles are suitable for
sets of samples showed the vibration of Si-O-Si structure photocuring printing.

Volume 9 Issue 5 (2023) 374 https://doi.org/10.18063/ijb.767