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International Journal of Bioprinting Precise fabrication of engineered vascular networks

Figure 10. Implantation of scaffolds with engineered vasculature in ischemia model for 4 weeks. (A) H&E staining for the implanted scaffolds and
surrounding tissues. Scale bar = 1 mm. (B) Masson’s trichrome staining for the implanted scaffolds and surrounding tissues. Scale bar = 1 mm.
(C) Immunofluorescence staining of CD31 and α-SMA for the implanted scaffolds and surrounding tissues. Scale bar = 1 mm. (D) H&E staining
of the vasculature lumens within the (i) 1 × 1, (ii) 4 × 4, and (iii) 8 × 8 scaffolds. Scale bar = 50 μm; scale bar in the magnified images = 20 μm.
(E) Immunofluorescence staining of CD31 and α-SMA for the vasculature lumens within the (i) 1 × 1, (ii) 4 × 4, and (iii) 8 × 8 scaffolds. Scale bar = 50 μm;
scale bar in the magnified images = 20 μm.

funded by the China Postdoctoral Science Foundation Conflict of interest
(2020TQ0195/2021M702165/2022M722121), the National
Natural Science Foundation of China (NSFC) Research The authors declare no conflict of interest.
Grants (82072412/81772326/81902195), Postdoctoral Author contributions
Incentive Program of Shanghai Jiao Tong University School
of Medicine, Project of Shanghai Science and Technology Conceptualization: Xue Yang, Shuai Li, Jinwu Wang,
Commission (19XD1434200), Two-hundred Talent Kerong Dai
Support (20152224), Translational Medicine National Key Formal analysis: Xue Yang, Shuai Li
Science and Technology Infrastructure (Shanghai) Open Investigation: Xue Yang, Xin Sun, Lei Qiang, Yihao Liu
Project (TMSK-2020-118), and Translational Medicine Methodology: Xin Sun, Ya Ren
Innovation Project of Shanghai Jiao Tong University Resources: Jinwu Wang, Kerong Dai
School of Medicine (TM201613). Supervision: Shuai Li, Jinwu Wang, Kerong Dai
Writing – original draft: Xue Yang, Shuai Li

Volume 9 Issue 5 (2023) 50 https://doi.org/10.18063/ijb.749