Page 62 - IJB-9-5

International Journal of Bioprinting

RESEARCH ARTICLE
A new 3D-printed polylactic acid-bioglass

composite for bone tissue engineering induces
angiogenesis in vitro and in ovo

Simon Cichos , Eva Schätzlein , Nadine Wiesmann-Imilowski ,
3,4
1
2
Andreas Blaeser , Dirk Henrich , Johannes Frank , Philipp Drees ,
1
6
6
2,5
Erol Gercek , Ulrike Ritz *
1
1
1 Department of Orthopedics and Traumatology, University Medical Center Mainz, Mainz, Germany
2 Technical University of Darmstadt, Institute for BioMedical Printing Technology, Darmstadt,
Germany
3 Department of Otorhinolaryngology, University Medical Center Mainz, Mainz, Germany
4 Department of Oral and Maxillofacial Surgery, University Medical Center Mainz, Mainz, Germany
5 Technical University of Darmstadt, Centre for Synthetic Biology, Darmstadt, Germany
6 Department of Trauma, Hand and Reconstructive Surgery, Goethe University Frankfurt, Frankfurt
am Main, Germany
(This article belongs to the Special Issue: Advances in the Application of Bioprinted Biomaterials in Tissue
Engineering)
Abstract
Large bone defects such as those that occur after trauma or resections due to cancer
*Corresponding author:
Ulrike Ritz (ritz@uni-mainz.de) still are a challenge for surgeons. Main challenge in this area is to find a suitable
alternative to the gold-standard therapy, which is highly risky, and a promising option is
Citation: Cichos S, Schätzlein E,
Wiesmann-Imilowski N, et al., 2023, to use biomaterials manufactured by 3D printing. In former studies, we demonstrated
A new 3D-printed polylactic acid- that the combination of polylactic acid (PLA) and bioglass (BG) resulted in a stable
bioglass composite for bone tissue 3D-printable material, and porous and finely structured scaffolds were printed. These
engineering induces angiogenesis
in vitro and in ovo. Int J Bioprint, scaffolds exhibited osteogenic and anti-inflammatory properties. This 3D-printed
9(5): 749. material fulfills most of the requirements described in the diamond concept of bone
https://doi.org/10.18063/ijb.751 healing. However, the question remains as to whether it also meets the requirements
Received: February 03, 2023 concerning angiogenesis. Therefore, the aim of this study was to analyze the effects
Accepted: March 04, 2023 of the 3D-printed PLA-BG composite material on angiogenesis. In vitro analyses
Published Online: May 11, 2023 with human umbilical vein endothelial cells (HUVECs) showed a positive effect of
Copyright: © 2023 Author(s). increasing BG content on viability and gene expression of endothelial markers.
This is an Open Access article This positive effect was confirmed by an enhanced vascular formation analyzed by
distributed under the terms of the
Creative Commons Attribution Matrigel assay and chicken chorioallantoic membrane (CAM) assay. In this work, we
License, permitting distribution, demonstrated the angiogenic efficiency of a 3D-printed PLA–BG composite material.
and reproduction in any medium, Recalling the osteogenic potential of this material demonstrated in former work,
provided the original work is
properly cited. we manufactured a mechanically stable, 3D-printable, osteogenic and angiogenic
material, which could be used for bone tissue engineering.
Publisher’s Note: Whioce
Publishing remains neutral with
regard to jurisdictional claims in
published maps and institutional Keywords: 3D printing; Polylactic acid; Bioglass; Angiogenesis; Vascular formation;
affiliations. In ovo CAM assay

Volume 9 Issue 5 (2023) 54 https://doi.org/10.18063/ijb.751