Page 223 - IJB-9-6
P. 223
International Journal of Bioprinting Multi-Cellular tissues/organoids manufacturing strategies
The scaffold-free strategy, on the other hand, is better at 2. Lancaster MA, Knoblich JA, 2014, Organogenesis in a
assembling complex structures and MTOs but requires dish: Modeling development and disease using organoid
accurate space positioning of cell aggregate spheroids. technologies. Science, 345(6194): 1247125.
Integrating both strategies could address the challenges in https://doi.org/10.1126/science.1247125
organoid biofabrication. This review also introduces the 3. Clevers H, 2016, Modeling development and disease with
BMMm, which aims to achieve a patterned arrangement organoids. Cell, 165(7): 1586–1597.
of heterogeneous cell aggregate spheroids in space to
build larger-scale tissue or organoids with complex https://doi.org/10.1016/j.cell.2016.05.082
structures. The BMMs is being developed as an intelligent 4. Meran L, Tullie L, Eaton S, et al., 2022, Bioengineering human
biofabrication device tailored to the BMMm. It combines intestinal mucosal grafts using patient-derived organoids,
3D bioprinting and bioassembly technology to allow for fibroblasts and scaffolds. Nat Protoc, 18(1): 108–135.
fully automated fabrication of tissue or organoid models. https://doi.org/10.1038/s41596-022-00751-1
The BMMs are expected to enable a compact desktop 5. Carosio S, Barberi L, Rizzuto E, et al., 2013, Generation of
design and facilitate the manufacturing process of tissue ex vivo-vascularized muscle engineered tissue (X-MET).
or organoid models with automation, intelligence, process Sci Rep, 3(1): 1–9.
orientation, and high precision. The BMMm and BMMs
aim to improve the high reproducibility of research results, https://doi.org/10.1038/srep01420
facilitate the mass production of organoid models, and 6. Kratochvil MJ, Seymour AJ, Li TL, et al., 2019, Engineered
improve medical efficiency. materials for organoid systems. Nat Rev Mater, 4(9): 606–622.
https://doi.org/10.1038/s41578-019-0129-9
Acknowledgments
7. Hofer M, Lutolf MP, 2021, Engineering organoids. Nat Rev
None. Mater, 6(5): 402–420.
Funding https://doi.org/10.1038/s41578-021-00279-y
8. Groll J, Boland T, Blunk T, et al., 2016, Biofabrication:
None. Reappraising the definition of an evolving field.
Biofabrication, 8(1): 013001.
Conflict of interest
https://doi.org/10.1088/1758-5090/8/1/013001
The authors declare no conflict of interest.
9. Heinrich MA, Liu W, Jimenez A, et al., 2019, 3D-Bioprinting:
Author contributions From benches to translational applications. Small, 15(23):
1805510.
Conceptualization: Haoyu Li, Huixing Zhou https://doi.org/10.1002/smll.201970126
Investigation: Haoyu Li, Huixing Zhou
Writing – original draft: Haoyu Li, Huixing Zhou 10. Moroni L, Burdick JA, Highley C, et al., 2018, Biofabrication
Writing – review & editing: Huixing Zhou, Chongwen Xu, strategies for 3D in vitro models and regenerative medicine.
Nat Rev Mater, 36(4): 21–37.
Yen Wei, Xiuying Tang
11. Martin I, Malda J, Rivron NC, 2019, Organs by design:
Ethics approval and consent to participate Can bioprinting meet self-organization? Curr Opin Organ
Transplant, 24(5): 562–567.
Not applicable.
https://doi.org/10.1097/MOT.0000000000000679
Consent for publication 12. Kačarević Ž, Rider P, Alkildani S, et al., 2018, An
Not applicable. introduction to 3D-bioprinting: Possibilities, challenges and
future aspects. Materials, 11(11): 2199.
Availability of data https://doi.org/10.3390/ma11112199
Not applicable. 13. Ahn CB, Lee J-H, Kim JH, et al., 2022, Development of a 3D
subcutaneous construct containing insulin-producing beta
References cells using bioprinting. Bio-Des Manuf, 5(2): 265–276.
https://doi.org/10.1007/s42242-021-00178-9
1. Prior N, Inacio P, Huch M, 2019, Liver organoids: From basic 14. Chen EP, Toksoy Z, Davis BA, et al., 2021, 3D-bioprinting
research to therapeutic applications. Gut, 68(12): 2228–2237.
of vascularized tissues for in vitro and in vivo applications.
https://doi.org/10.1136/gutjnl-2019-319256 Front Bioeng Biotechnol, 9: 664188.
Volume 9 Issue 6 (2023) 215 https://doi.org/10.36922/ijb.0135

