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International Journal of Bioprinting 3D bioprinting of in vitro cartilage tissue model

feasibility to use this material as a bioink and as a short- Writing – original draft: Patricia Santos-Beato
term culture platform to develop human cartilage models Writing – review & editing: Andrew A. Pitsillides, Alberto
in vitro. This material, as a bioprintable material to develop Saiani, Aline Miller, Deepak M. Kalaskar
human cartilage models, can be applied in personalized
medicine, fundamental research, or disease modeling. Ethics approval and consent to participate
Not applicable.
5. Conclusion
In this study, we optimized the bioprinting process of Consent for publication
PeptiInk Alpha 1 and demonstrated its potential to Not applicable.
manufacture human cartilage models in vitro. First,
we assessed the printability of the material through Availability of data
rheological characterization and optimization of printing
pressures and speeds using a 25G conical nozzle. We Raw data can be accessed by contacting corresponding
went on to explore the behavior that primary human author
chondrocytes show when encapsulated and 3D-bioprinted
within Alpha 1. High cell viability, cell self-assembly, Further disclosure
and chondrogenic protein expression at the protein and Part of the entire set of findings has been presented
mRNA levels were observed in both the control and the in a conference (European Society of Biomaterials,
PeptiInk Alpha 1 culture. This material, which can be September 2022).
potentially used to 3D-bioprint human cartilage tissue
models in vitro, presents a more ethical and sustainable References
alternative than the current 3D-bioprinted cartilage
in vitro models. Further work will focus on additional 1. Gibofsky A, 2012, Overview of epidemiology,
assessment of the chondrogenic behavior through ELISA pathophysiology, and diagnosis of rheumatoid arthritis. Am
protein quantification, improvement of the material J Manag Care, 18(13 Suppl): S295–S302.
stability for long-term cultures, and 3D bioprinting of 2. Jafarzadeh SR, Felson DT, 2018, Updated estimates suggest
larger constructs. a much higher prevalence of arthritis in United States adults
than previous ones. Arthritis Rheumatol, 70(2): 185–192.
Acknowledgments
https://pubmed.ncbi.nlm.nih.gov/29178176
This article was written and revised only by the confirmed 3. Buckwalter JA, Mow VC, Ratcliffe A, 1994, Restoration of
authors, and no other people contributed to the production injured or degenerated articular cartilage. J Am Acad Orthop
of this paper. Surg, 2(4): 192–201.
Funding 4. O’Hara BP, Urban JP, Maroudas A, 1990, Influence of cyclic
loading on the nutrition of articular cartilage. Ann Rheum
This work was supported by the Engineering and Physical Dis, 49(7): 536–539.
Sciences Research Council (EPSRC- EP/S021868/1) Centre 5. Chang AA, Reuther MS, Briggs KK, et al., 2012, In vivo
for Doctoral Training. implantation of tissue-engineered human nasal septal
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Conflict of interest Neck Surg, 146(1): 46–52.
The authors declare no conflict of interest. https://pubmed.ncbi.nlm.nih.gov/22031592
6. Jin CZ, Cho JH, Choi BH, et al., 2011, The maturity of tissue-
Author contributions engineered cartilage in vitro affects the repairability for
osteochondral defect. Tissue Eng Part A, 17(23–24): 3057–
Conceptualization: Patricia Santos-Beato, Andrew A. 3065.
Pitsillides
Formal analysis: Patricia Santos-Beato https://pubmed.ncbi.nlm.nih.gov/21736425
Funding acquisition: Deepak M. Kalaskar 7. Neybecker P, Henrionnet C, Pape E, et al., 2018, In vitro
Investigation: Patricia Santos-Beato and in vivo potentialities for cartilage repair from human
Supervision: Andrew A. Pitsillides, Alberto Saiani, Aline advanced knee osteoarthritis synovial fluid-derived
Miller, Ryo Torii, Deepak M. Kalaskar mesenchymal stem cells. Stem Cell Res Ther, 9(1): 329.
Visualization: Patricia Santos-Beato https://doi.org/10.1186/s13287-018-1071-2

Volume 9 Issue 6 (2023) 463 https://doi.org/10.36922/ijb.0899