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International Journal of Bioprinting 3D bioprinting of in vitro cartilage tissue model

Figure 5. Top: Collagen type II staining (green) and cell nuclei staining with DAPI (blue) in 3D chondrocyte pellet and PeptiInk Alpha 1 at days 7 and 14.
Bottom: Aggrecan staining (red) and cell nuclei staining with DAPI (blue) of 3D chondrocyte pellet and PeptiInk Alpha 1 at days 7 and 14.

the theoretical filament width, which corresponds to the to manually change the printing pressure, within this
printing nozzle diameter. As expected, the higher was the window, to avoid loss of structural consistency. Higher
printing speed, the thinner was the deposited filament, printing speeds did not allow for consistent and fast-
and the closer was the resolution to the theoretical optimal enough pressure changes that would ensure the required
value. Although the printing speed of 10 mm/s at 8 kPa structural integrity. 3D bioprinting as the preferred choice
of pressure showed the best printing resolution, a lower of manufacturing technique overall demonstrated ease of
printing speed was chosen for the final manufacturing of use, consistency when manufacturing these constructs,
the constructs to avoid potential printing issues. Due to and the possibility of scaling up this manufacturing
the constant pressure changes that the printer experienced process to produce more 3D-bioprinted constructs for
when moving from one well to another within the well future cartilage modeling in vitro.
plate, a slower printing speed enabled the printer user

Volume 9 Issue 6 (2023) 460 https://doi.org/10.36922/ijb.0899