Page 474 - IJB-9-6

International

Journal of Bioprinting

REVIEW ARTICLE
Bioprinting of cell-laden protein-based

hydrogels: From cartilage to bone tissue
engineering

Mehran Khajehmohammadi 1,2† , Negar Bakhtiary , Niyousha Davari ,
3†
4
Soulmaz Sarkari , Hamidreza Tolabi , Dejian Li , Behafarid Ghalandari *,
5
8
9
6,7
Baoqing Yu *, and Farnaz Ghorbani 11,12,13 *
10
1 Department of Mechanical Engineering, Faculty of Engineering, Yazd University, Yazd 8174848351,
Iran
2 Medical Nanotechnology and Tissue Engineering Research Center, Yazd Reproductive Sciences
Institute, Shahid Sadoughi University of Medical Sciences, Yazd 8916877391, Iran
3
Department of Biomaterials, Faculty of Interdisciplinary Science and Technology, Tarbiat Modares
University, Tehran 14115114, Iran
4 Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of
Tehran, Tehran 143951561, Iran
5 Department of Cardiology, School of Medicine and Health Sciences, Carl von Ossietzky University
of Oldenburg, Oldenburg, Germany
6 New Technologies Research Center (NTRC), Amirkabir University of Technology, Tehran
† These authors contributed equally 158754413, Iran
to this work.
7 Department of Biomedical Engineering, Amirkabir University of Technology (Tehran Polytechnic),
*Corresponding authors: Tehran 158754413, Iran
Behafarid Ghalandari 8 Department of Orthopedics, Shanghai Pudong Hospital, Fudan University Pudong Medical Center,
(behafarid.gh@gamil.com
Shanghai, China
Baoqing Yu 9 State Key Laboratory of Oncogenes and Related Genes, Institute for Personalized Medicine,
(doctorybq@163.com) School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China
Farnaz Ghorbani 10 Department of Orthopedics, Shanghai Pudong New Area People’s Hospital, Shanghai, China
(farnaz.ghorbani@fau.de) 11 Department of Translational Health Sciences, University of Bristol, Bristol BS1 3NY, United
Citation: Khajehmohammadi M, Kingdom
Bakhtiary N, Davari N, et al., 2023, 12 Institute of Biomaterials, Department of Material Science and Engineering, University of Erlangen-
Bioprinting of cell-laden protein- Nuremberg, Cauerstraße 6, 91058 Erlangen, Germany
based hydrogels: From cartilage
to bone tissue engineering. Int J 13 Institute of Orthopaedic and Musculoskeletal Science, Royal National Orthopaedic Hospital,
Bioprint, 9(6): 1089. University College London, London, United Kingdom
https://doi.org/10.36922/ijb.1089
(This article belongs to the Special Issue: Advances in 3D Bioprinting for Regenerative Medicine and Drug
Received: June 16, 2023 Screening)
Accepted: August 3, 2023
Published Online: September 7,
2023 Abstract
Copyright: © 2023 Author(s).
This is an Open Access article The fabrication of cell-laden protein-based hydrogels (PBHs) for bioprinting necessitates
distributed under the terms of the careful consideration of numerous factors to ensure optimal structure and functionality.
Creative Commons Attribution Bioprinting techniques, such as single-cell, multi-cell, and cell aggregate bioprinting,
License, permitting distribution,
and reproduction in any medium, are employed to encapsulate cells within PBHs bioink, enabling the creation of scaffolds
provided the original work is for cartilage and bone regeneration. During the fabrication process, it is imperative to
properly cited. account for biophysical and biochemical factors that influence cell behavior and protein
Publisher’s Note: AccScience structure within the PBHs. Precise control of crosslinking methods, hydrogel rheological
Publishing remains neutral with properties, and printing parameters is also crucial to achieve desired scaffold properties
regard to jurisdictional claims in without compromising cell viability and protein integrity. This review primarily focuses
published maps and institutional
affiliations. on the influence of biophysical factors, including composition, microstructure,

Volume 9 Issue 6 (2023) 466 https://doi.org/10.36922/ijb.1089