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International Journal of Bioprinting Osteocytic Wnt7b-PKCδ against microgravity

Figure 5. Osteogenic and anti-adipogenic effects of osteocytic Wnt7b in 3D modules. (A–C) AP staining (A), AP activity (B), and osteoblast marker
gene expression (C) of Y4-Wnt7b on osteoblast differentiation of ST2 cells in PCI3D modules cultured for 7 and 14 days. (D) Alizarin Red S staining of
mineralization. (E) Adipogenic gene expression (Pparg, Cebpa, Fabp4). *p < 0.05 versus Y4-GFP by Student’s t-test; n = 3.

Volume 11 Issue 4 (2025) 438 doi: 10.36922/IJB025240238