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International Journal of Bioprinting Osteocytic Wnt7b-PKCδ against microgravity

regulator Mef2c. Furthermore, elevated pro-inflammatory mechanotransduction cascades in microgravity likely
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cytokines (Tnfa, Il1b) are consistent with the inflammatory impairs normal osteocyte function, biasing stromal cell
and catabolic state triggered by microgravity, potentially
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contributing to enhanced osteoclastogenesis, as evidenced fate towards adipogenesis at the expense of osteogenesis.
by an increased Rankl/Opg ratio. The disruption of Thus, the 3D-BWBM system robustly recapitulates key

Figure 7. Osteocytic Wnt7b protects osteogenesis under microgravity. (A–C) Alkaline phosphatase (AP) staining (A), AP activity (B), and osteoblast
marker gene expression (C) in PCI3D modules cultured in 3D-BWBM for 7 days. (D) Adipogenic gene expression under microgravity conditions.
(E) Proposed model: Wnt7b-PKCδ signaling bypasses sclerostin-mediated inhibition of canonical Wnt to promote osteogenesis. *p < 0.05 vs. Y4-GFP;
n = 3. Abbreviation: 3D-BWBM, 3D biomimetic weightless biomicroenvironmental system.

Volume 11 Issue 4 (2025) 440 doi: 10.36922/IJB025240238