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International Journal of Bioprinting Osteocytic Wnt7b-PKCδ against microgravity
Figure 6. The 3D-BWBM system for microgravity simulation. (A) Diagram of the 3D-BWBM system combining PCI3D printing (Part A) and NASA’s
RCCS™ bioreactor (Part B). (B) Reverse transcription quantitative polymerase chain reaction (RT-qPCR) results of Sost (sclerostin), Mef2c, Il1b, Tnfa, and
Il8 in PCI3D modules under static or microgravity conditions. *p < 0.05 versus Y4-GFP by Student’s t-test; n = 3. Abbreviations: 3D-BWBM, 3D biomimetic
weightless bio-microenvironmental system; NASA, National Aeronautics and Space Administration; PCI3D, PCL and cell-integrated 3D-printed; RCCS™,
Rotary Cell Culture System; RT-qPCR, reverse transcription quantitative polymerase chain reaction.
Volume 11 Issue 4 (2025) 439 doi: 10.36922/IJB025240238
