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2 INNOSC Theranostics and Pharmacological Sciences, 2023, Vol. 6, No. 1 Vishwakarma, et al.
peroxidases. The factors that generate reactive antioxidant assays such as β-carotene-linoleic acid
oxygen species (ROS) are both from the products of reduction, 1,1-diphenyl-2-picrylhydrazyl (DPPH)
normal cellular physiology as well as from various free radical scavenging activity, and ferrous
exogenous sources. The excessive production chelating ability. It was further established that
of ROS and reactive nitrogen species (RNS) in P. ostreatus has antioxidant properties by virtue of
or around the pancreatic beta cells is the major its phenolic composition and being enriched with
physiological reason that causes beta cells death selenium and zinc which serve as antioxidants [10].
and deficiency in insulin [3]. The development of Thus, this study aimed to utilize the antioxidant
insulin-dependent diabetes mellitus type 1 can be potential of P. ostreatus in an experimental model
triggered by several risk factors, such as genetic, of diabetes. Streptozotocin (STZ), chemically an
developmental, environmental and dietary factors. N-nitroso derivative of glucosamine, is a vast-
However, the ROS/RNS play central role in spectrum antibiotic derived from Streptomyces
pancreatic β-cell death and disease progression, chromogens. It acts as a pancreatic beta cell toxoid
and both of the radical species play critical roles in that stimulates and accelerates irreversible necrosis
cellular autoimmune-inflammatory responses [4]. of pancreatic beta cells, making it an extensively
Therefore, specific treatments with antioxidants and used chemical inducer of diabetes in experimental
inflammatory drugs may inhibit the hyperglycemic animal models [11]. Thus, the present study
response. determined the antidiabetic activity of an aqueous
At present, treatments of diabetes mellitus extract of P. ostreatus (AEPO) in an experimental
include exercise, diet therapy, insulin therapy and model of diabetes induced by STZ in rats.
oral antidiabetic agents such as sulfonylureas,
biquanides, thiazolidinediones, and alpha 2. Materials and methods
glucosidase inhibitor. Despite of their effectiveness 2.1. Collection and preparation of AEPO
in reducing hyperglycemia, the use of these drugs
is associated with non-desirable side effects [5]. P. ostreatus was collected from Gorakhpur district,
Hence, herbal medicine can be used as an Uttar Pradesh, India. The sample was identified
alternative therapy for treatment of diabetes. These by relevant literature [12] and the sample was
herbal medicines help to reduce the complications submitted with voucher number DDUNPL250 to
associated with drugs and thus help in maintaining the institutional repository. For extraction purpose,
normal glucose level without triggering any the fruiting bodies were dried under shade and
complications [6]. Mushrooms are important food ground to fine powder with the help of a grinder and
sources and represent a vast, untapped source of stored in opaque screw top jar at room temperature.
natural pharmaceutical products [7]. Mushrooms Aqueous extract of the macrofungal samples was
have low glycemic index which makes them low- prepared by the method of infusion. One gram
calorie food, and contain high nutrients such as of macrofungal sample as powder was mixed in
protein; therefore, they can be recommended for 40 mL of boiling distilled water and allowed to
diabetics [7]. Pleurotus spp. or oyster mushrooms infuse for 15 min. Then, it was filtered by Whatman
are rich in medicinal values such as effectiveness no. 1 filter paper, and the volume was readjusted to

in reducing the total plasma cholesterol and 40 mL[13]. The final preparation named as AEPO
triglyceride level and thus may reduce the chance of was stored at 4°C until utilization.
atherosclerosis and other cardiovascular and artery- 2.2. Evaluation of antidiabetic potential in
related disorders [8]. These medicinal properties experimental model of diabetes in rats
might be due to the presence of some important
substance in dietary mushrooms [8]. Pleurotus In vivo experiment was designed according to
ostreatus possessed potent antioxidant effects in method adopted by Kim et al. [14] with few
the supplementation of corncobs with different modifications. The protocols for these experiments
herbs [9]. The antioxidant activity of P. ostreatus were approved by the Animal Ethical Committee of
was found to be mechanistically associated with the Institute (IAEC/DDU/2021-22). Healthy adult
tyrosinase inhibitory effects tested in several male albino rats weighing approximately 60–160 g

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