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5 INNOSC Theranostics and Pharmacological Sciences, 2023, Vol. 6, No. 1 Vishwakarma, et al.
STZ-diabetic rats showed a reducing trend in the were about 3 – 20% reductions, whereas AEPO at
level of blood glucose by 170, 159, and 130 mg/dL 200 mg/kg dose led to a reduction oft blood glucose
at 24, 48, and 72 h, respectively. These reductions in levels to 175, 161, and 139 mg/dL at 24, 48, and
the blood glucose levels accounted for 12%, 17%, 72 h, respectively, with about 7 – 26% reductions.
and 32% at 24, 48, and 72 h, respectively. The data AEPO at 200 mg/kg dose showed a blood glucose-
were significantly lowered (P < 0.05) as compared lowering effect in STZ-diabetic rats similar to that of
to vehicle control at different time points. AEPO metformin (500 mg/kg). Thus, these results showed
at each concentration tested showed substantial potent antihyperglycemic effects of P. ostreatus in
results in lowering the blood glucose levels. Oral diabetic rats.
administration of AEPO to STZ-induced diabetic
rats showed a dose- and time-dependent decrease 3.3. Postprandial antihyperglycemic effects of
in the blood glucose levels. AEPO (100 mg/kg) P. ostreatus on STZ-induced diabetic rats: acute
treatment to STZ-diabetic rats showed that blood and chronic assessment
glucose levels were reduced to 177, 165, and Postprandial blood sugar levels are important
145 mg/dL at 24, 48, and 72 h, respectively, which indicator for diabetes and hyperglycemia, which is
tested along with fasting blood sugar. Postprandial
blood sugar measurements can give a clue about the
metabolic health in a metabolically altered state of
diabetes [23]. For postprandial antihyperglycemic
test, two different criteria were used; first as acute
study performed at different short intervals of
0, 2, 4, 6, and 24 h, and second as chronic study
performed at 0, 5, 10, 15 and 30 days. In the acute
study (Table 2), rats in vehicle control group
showed a normal course of decline in postprandial
blood glucose levels from 0 h to 24 h with total
Figure 2. Effect of aqueous extract of Pleurotus about 25 mg/dL reduction in 24 h, whereas STZ-
ostreatus and metformin on body weight of diabetic induced diabetic rats showed an increasing trend
rats. Values are mean ± standard error of mean for in postprandial blood glucose levels from 0 h
groups of 3 observations with their standard errors. (270 ± 1.65 mg/dL) to 24 h (310 ± 2.92 mg/dL)
↓ decrease; ↑ increase in body weight. *P<0.05 with total about 40 mg/dL (14.8%) increment in
vs vehicle control 30 days; P<0.05 vs. within 24 h. Treatment of STZ-induced diabetic rats with
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group 30 min. metformin (500 mg/kg) caused a time-dependent

Table 1. Effect of AEPO and metformin on fasting glucose level in STZ- induced diabetic rats
Experimental groups Blood glucose level (mg/dL)
Before treatment 24 h after treatment 48 h after treatment 72 h after treatment
Vehicle control 99±2.32 95±1.95 98±2.77 98±2.98
STZ-induced (diabetic) 190±1.38* 193±2.84* 195±2.37* 195±2.85*
Diabetic+metformin (500 mg/kg) 193±2.33* 170±2.37 # 159±1.19 # 130±3.65 #
^11.92% ^17.61% ^32.64%
Diabetic+AEPO (100 mg/kg) 182±2.72* 177±1.39 # 165±0.99 # 145±3.75 #
^2.74% ^9.34% ^20.33%
Diabetic + AEPO (200 mg/kg) 188±2.84* 175±1.33 # 161±1.37 # 139±1.72 #

^6.91% ^14.36% ^26.06%
Values are mean±SEM for groups of three observations with their standard errors. AEPO: Aqueous extract of Pleurotus ostreatus;
STZ: Streptozotocin. ^Values in percent indicate reductions in the blood glucose levels relative to 0 h (before treatment) of the respective
treatment groups. *P<0.05 versus control; P<0.05 versus STZ group
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