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4 INNOSC Theranostics and Pharmacological Sciences, 2023, Vol. 6, No. 1 Vishwakarma, et al.

Figure 1. Schematic diagram for collection and preparation of the aqueous extract of Pleurotus ostreatus.

Metformin and AEPO were given to experimental no harmful effects observed in rats due to treatment
rats every day. with AEPO, showing that it caused no changes
in gross behavior of animals. Data presented in
2.7. Hematological assessment Figure 2 demonstrated that control rats were

Hematological analysis was performed from whole more stable in their body weight. STZ-treated
blood sample using the Hematological Analyzer diabetic rats showed significant reduction in weight
Sysmex (KX 21, Japan). Erythrocyte count (red (39%) as compared to vehicle control (P < 0.05).
blood cell [RBC]), hemoglobin concentration Metformin-treated diabetic rats showed a greater
(Hb), leukocyte count (white blood cell [WBC]), effect on body weight with 17.3% reduction in body
neutrophil percentage, and lymphocyte percentage weight (P < 0.05). AEPO treatment to diabetic rats
in WBC were measured in control and treated rats. caused a dose-dependent increase in body weight
Biochemical Analyzer (Transasia ERA CHEM-5 by 16.3% and 20.2% increase in body weight at 100
PLUS, India) was used for analyzing blood and 200 mg/kg dosage, respectively. Diabetic rats
biochemical parameters. treated with AEPO (200 mg/kg) showed a notable
effect that helped to increase the body weight of
2.8. Statistical analysis experimental diabetic rat with a similar response

Data are presented as mean ± standard deviation to metformin (Figure 1). These results showed a
(SD) or standard error of mean (SEM) from preliminary protective effect of AEPO in diabetic
minimum three experimental repeats. The data were rats as physiological marker of health.
statistically analyzed by Students t-test and analysis 3.2. Effect of P. ostreatus on fasting blood glucose
of variance to compare parameters among groups. level in STZ-induced diabetic rats
P < 0.05 was considered statistically significant.
Reduction in blood glucose level is considered
3. Results the biochemical marker of antidiabetic effect

3.1. Effect of P. ostreatus on body weight of test of a candidate drug agent. As it is evident
animals from Table 1, fasting blood glucose level was
elevated to a notable level (190 ± 1.38 mg/dL)
Body weight and behavioral change of the in diabetic rats in comparison to vehicle control
experimental animals were monitored as group (99 ± 2.32 mg/dL) at the time of starting the
physiological markers of the health. Data showed experiment (before treatment stage). STZ-induced
that oral administration of AEPO caused no diabetic rats showed a narrow range of blood
changes in gross behavior, and none of the animals glucose level (182 – 193 mg/dL) at the time of
died during the course of experiment. There were starting the experiment. Metformin treatment to

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