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3 INNOSC Theranostics and Pharmacological Sciences, 2023, Vol. 6, No. 1 Vishwakarma, et al.
were obtained and were kept at room temperature monitoring system). Animals with blood glucose
25±5 C in large cage with polypropylene-coated level higher than 180 mg/dL were considered
o
wire gauze on all sides. Rats were exposed to a diabetic and used for the experiment.
photoperiod of 12 h/day. The cages were cleaned
regularly to avoid rat smell and to maintain proper 2.4. Assessment of body weight
hygienic conditions. The rats were acclimatized to Rats were weighed initially and after the
laboratory conditions for 10 days and fed on rat experiment. The relative change in body weight
pellets and water ad libitum. Each rat was weighed (b.w.) of rat was determined in percentage using
and assigned a number for convenience before the following equation:
onset of experiment. Experimental animals were
divided into five groups with three rats in each Percent relative change in b.w.
(n=3); the categorization plan is as follows: = Pre-treatment b.w. – Post-treatment b.w. × 100
(i) Group 1: Vehicle control (received 0.1 M citrate Pre-treatment b.w.
buffer, i.p.)
(ii) Group 2: Diabetic rat (received STZ 50 mg/kg
b.w. in 0.1 M citrate buffer, i.p., single dose) 2.5. Oral glucose tolerance test (OGTT)
(iii) Group 3: Diabetic rat received metformin The experimental animals were fasted overnight
(500 mg/kg b.w. aqueous solution, p.o., per day, (about 12 h) before commencing the experiment.
for 4 weeks) Rats of all the groups were given D-glucose
(iv) Group 4: Diabetic rat received AEPO (100 mg/ (500 mg/mL) solution after half an hour after
kg b.w., p.o., per day, for 4 weeks) metformin and AEPO administration. Blood
(v) Group 5: Diabetic rat received AEPO (200 mg/ samples were withdrawn from tail vein before and
kg b.w., p.o., per day, for 4 weeks) after the metformin and AEPO administration at
30, 60, 90, and 120 min.
Studies have utilized a dose range of 100–
500 mg/kg for investigating the biomedicinal effects 2.6. Determination of serum glucose
of P. ostreatus against several disease models in Blood glucose level was measured in two steps;
rats [15-17]. Based on above observations and our first, fasting blood glucose level was measured, and
previous studies with 100–300 mg/kg mushroom second, postprandial antihyperglycemic was tested.
extracts [18-22] with no notable toxicity, we used
100 and 200 mg/kg AEPO in this study. STZ was 2.6.1. Fasting blood glucose level
administered as a single dose while metformin and Experimental animals were deprived of food for about
AEPO per day for 4 weeks. 16 h with free access to drinking water before the

2.3. Induction of diabetes in rats commencement of experiment. Experimental animals
were tested for their blood glucose level at 0, 24, 48,
Diabetes mellitus in rats was induced by single and 72 h of treatment of AEPO. Blood samples were
intraperitoneal (i.p.) administration of freshly collected from the tail vein for glucose analysis.
prepared solution of STZ (HiMedia, CMS 1758) 2.6.2. Postprandial antihyperglycemic test
dissolved in 0.1 M citrate buffer, pH = 4.5 (vehicle
control). For diabetes induction, each test animal This study was done in two steps, that is, acute and
was injected with 50 mg/kg volume of freshly chronic. For acute study, rats were fasted for 1 h
prepared STZ. At the same time, normal rats before test. After the fasting period, rats were given
received the same volume of vehicle control either metformin or AEPO orally using intragastric
through the same route. The animals were returned gavage. Blood samples were collected from the tail
to their cages after injection and allowed for free vein just before (0 h) and after 2, 4, 6, and 24 h
access to food and water. After 3–4 days, the fasting administration of metformin or AEPO. In chronic
blood glucose level was measured from the tail vein study, glucose level in blood was measured at
using glucometer (mylife Pura blood glucose 0 day and 5, 10, 15, and 30 days after treatment.

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