Page 75 - ITPS-7-4

INNOSC Theranostics and
Pharmacological Sciences Estrogen treatment reverse hypertension

uterine horn, where the horn was cut to excise the ovary. acid reactive substances assay. Markers of oxidative
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The uterus and fat pad were then returned to the abdominal stress, including reduced glutathione (GSH), catalase
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cavity, and the muscle and skin incisions were sutured with (CAT), and superoxide dismutase (SOD). activities
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4/0 absorbable sutures (Chromic Catgut Suture, Nigeria). were determined using previously reported methods.
The same procedure was used to remove the contralateral
ovary. After surgery, the suture sites were cleaned and 2.7. Statistical analysis
treated with penicillin to prevent infection. The animals All data were summarized as mean ± standard error of the
were administered ofloxacin (100 mg/kg orally) for 7 days mean. The normality of the data was confirmed, followed
to prevent infection. Following surgery, the rats were by a one-way analysis of variance (ANOVA). Bonferroni’s
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allowed a 1-week recovery period with unrestricted access multiple comparisons test was applied for post-hoc analysis.
to food and water. A further week was allowed for the The confidence level was set at 95%.
rats to attain a post-menopausal state before the start of
treatment and diet administration. 3. Results

The study consisted of 10 groups, each containing 3.1. ETD and antihyperlipidemic drugs improve
six rats. The first group was the Sham group, which blood pressure parameters in OVX rats treated with
underwent a simulated surgery for surgical stress and thermoxidized oils
was fed normal rat chow. The other nine groups were Blood pressure parameters, including SBP, DBP, and MAP,
ovariectomized (OVX) and fed the test diets containing were not significantly affected in OVX rats compared to
TPO or TSO. Group 2 received the TSO diet, and Group 3 the Sham group. However, SBP and MAP were markedly
received the TPO diet. The remaining treatment groups elevated in OVX + TSO rats compared to Sham rats
were fed either the TSO or TPO diet and concurrently (Table 1). Treatment with EZE + ATV reduced SBP
treated with one of the following: atorvastatin (ATV) at (P < 0.05) compared to the untreated OVX+TSO group.
10 mg/kg/day, a combination of ezetimibe (EZE) and ATV
(EZE at 3 mg/kg/day + ATV at 10 mg/kg/day), or estradiol In OVX+TPO rats, there were significant increases
valerate (ETD) at 0.2 mg/kg/day. The diets and treatments in SBP, DBP, and MAP compared to Sham rats (Table 2).
were administered concurrently for 12 weeks, after which However, treatment with ATV, EZE+ATV, and ETD
blood pressure parameters were measured. normalized the SBP, DBP, and MAP parameters in these
rats.
2.4. Measurement of blood pressure and
electrocardiogram (ECG) parameters 3.2. ETD and antihyperlipidemic drugs improve ECG
parameters in OVX rats treated with thermoxidized
Systolic (SBP) and diastolic blood pressure, as well as oils
mean arterial pressure (MAP), were measured using a
non-invasive tail-cuff sphygmomanometer (CODA Tail Heart rate (Figure 1A), QRS duration (Figure 1B), QT
Cuff Blood Pressure Monitor, Kent Scientific, United States interval (Figure 1C), and QTc (Figure 1D) values were
of America). ECG parameters, including PR interval, Table 1. Effects of estradiol and antihyperlipidemic drugs on
QT interval, heart rate, QRS duration, P duration, R blood pressure parameters in ovariectomized Wistar rats fed
amplitude, and QT corrected (QTc), were measured using thermoxidized soya oil
an electrocardiograph machine (VE 1010 Veterinary
6/7 Channel PC ECG Machine, EDAN, United States of Treatment Blood pressure parameters (mmHg) a
America). SBP DBP MAP
Sham 107.30±3.87 78.25±3.10 87.46±2.09
2.5. Serum lipid profile analyses
OVX 109.20±3.02 83.87±2.15 90.06±1.48
Serum total cholesterol, triglycerides, and HDL-c were OVX+TSO 138.30±1.91 # 98.58±2.99 111.50±2.41 #
measured using specialized kits from Randox (Randox OVX+TSO+ATV 130.00±4.16 95. 88±4.61 106.9±4.25
Laboratories, United Kingdom). The concentrations of
LDL and very LDL (VLDL), as well as the atherogenic OVX+TSO+EZE+ATV 111.80±7.10* 83.84±7.82 92.91±7.59
index (AI), coronary risk index (CRI), and HDL-c/LDL-c OVX+TSO+ETD 130.00±11.02 92.45±10.02 104.7±9.83
ratio, were calculated using established formulas. 18-20 Notes: Values are presented as mean±SEM. Statistical significance:
a
# P<0.05 compared to Sham; *P<0.05 compared to OVX+TSO.
2.6. Serum oxidative stress assessment Abbreviations: ATV: Atorvastatin; DBP: Diastolic blood pressure;
ETD: Estradiol valerate; EZE: Ezetimibe; MAP: Mean arterial
Lipid peroxidation was assessed by measuring pressure; OVX: Ovariectomized; SBP: Systolic blood pressure;
malondialdehyde (MDA) levels using the thiobarbituric TSO: Thermoxidised soya oil.

Volume 7 Issue 4 (2024) 3 doi: 10.36922/itps.3901