Page 50 - JCBP-2-3

Journal of Clinical and
Basic Psychosomatics Proteomic analysis of mind-body psychotherapy in psoriasis

1. Introduction transferred by blood flow to the skin cells, impacting
their communications, thereby unfavorably resulting in
Psoriasis is a recurrent chronic autoimmune skin disease lesions. Therefore, exosomes are regarded as desirable
16
that affects the quality of life of patients. Genetic diagnostic and therapeutic biomarkers for skin diseases.
1
predisposition, infection, endocrine environment, Furthermore, exosomes from psoriasis-like keratinocytes
immunity, and metabolic disorders are etiologically related enhance the production of neutrophil extracellular
with psoriasis; however, the exact pathogenesis remains traps formation and proinflammatory cytokine
poorly understood. It is known that 44% of psoriasis expression through activating the nuclear factor kappa
1,2
patients endure psychological stress before the disease 17
onset, and 88% of them are subject to recurrent skin flares. B and p38 MAPK pathway. Thus, exosome-mediated
3
Relaxation, hypnosis, biofeedback, and behavioral and communication between activated keratinocytes and
cognitive stress management therapies have been proven infiltrating immune cells contributes to the progression
of psoriasis. Moreover, a previous study showed that
successful in treating patients with psoriasis. 4
the exosomes contain more intact proteins in culture
Abnormal proliferation of keratinocytes and immune supernatants of lipopolysaccharide-simulated THP-1 cells
dysregulation are the the main pathological manifestations than those from non-stimulated cells. 18
of psoriasis. At the cellular level, the skin lesions in
5
psoriasis exhibit hyperproliferation and aberrant growth Proteomics refer to the large-scale systematic analysis
of keratinocytes as well as large-extent infiltration by of proteins, in terms of identity, quantity, and function.
immune cells such as neutrophils, macrophages, T-cells, Biomarkers that have applications in the early and effective
and dendritic cells. Keratinocytes may stimulate T-cells, diagnosis, prognosis, and therapeutic outcome monitoring
6,7
respond to different stimuli, and play a pivotal role in the for psoriasis PS are urgently needed to optimize
inflammatory lesions of psoriasis; aberrantly released the diagnosis-prognosis-treatment axis of psoriasis
cytokines and chemokines from keratinocytes can enhance management. Over the past two decades, proteomics has
19
migration or expansion of the inflammatory cells, including been widely employed in psoriatic research. Using sera
neutrophils. These disorganized interactions between skin- from six psoriasis patients, four individual parameters such
resident immune cells and keratinocytes cause persistent as Zn-α2-glycoprotein, complement C3 (CO3), polymeric
immune-mediated inflammatory responses in psoriasis. immunoglobulin receptor, and plasma kallikrein (KLK)
8
Recently, exosomes have emerged as crucial mediators of were found to be differently expressed as compared to those
20
intercellular communication. Needless to say, exosomes, from healthy individuals. Proteomics analysis can also
9,10
along with inflammatory cytokines, together play a role help with differentiating responders and non-responders
21
in promoting the development of skin inflammation in to traditional Chinese medicine among psoriasis patients.
psoriasis by facilitating the interaction between immune However, studies on exploring the therapeutic impact of
cells and keratinocytes. 6 plasma exosome-derived proteins on psoriasis patients are
hitherto unavailable.
Exosomes are lipid-bilayer vesicles derived from
various cells, measuring 30 – 100 nm in diameter, and Mongolian mind-body interactive psychotherapy
11
found in various body fluids such as plasma, breast milk, (MMIP) is a new kind of complementary therapy that
urine, and saliva under pathological or non-pathological combines modern psychology with traditional Mongolian
conditions. Proteins associated with biogenesis (ALIX medicine theory, which has been introduced in literature. 22
12
and SG101), tetraspanins (CD81, CD63, and CD9), MMIP utilizes video or live group therapy approach (200
heat shock proteins (HSP70 and HSP90), and major – 1000 people), through which the therapist lectures about
histocompatibility complex (MHC-I and MHC-II) are knowledge of health, behavior, diet, mind, and psychology;
among the conserved proteins that are abundant in patients present their own case of recovery or the
exosomes. In addition to various proteins, exosomes significant therapeutic improvement their have attained;
13
also contain lipids and nucleic acids, including RNAs, and the therapist offers feedback about their recovery
miRNAs, lncRNAs, and circRNAs. These exosomes course or treatment path. MMIP is a form of group
participate in intricate, physiologically significant psychotherapy that does not apply medicine or drugs,
intercellular communication between various cells. featuring the concept of homotherapy-for-heteropathy.
14
The dysregulation of skin cell interactions associated This type of psychotherapy is less costly and associated
with alterations in exosome cargoes might play roles in with no side effects. Technically, MMIP utilizes narrative
the complex pathogenesis of chronic inflammatory and therapy, hypnosis therapy, music therapy, supportive
autoimmune skin diseases. During the development therapy, cognitive psychotherapy, positive psychotherapy,
15
of skin diseases, the cargoes in circulating exosomes are etc. 22,23 Our previous study showed that patients with

Volume 2 Issue 3 (2024) 2 doi: 10.36922/jcbp.2381