Page 50 - JCTR-9-6

Journal of Clinical and Translational Research 2023; 9(6): 414-422

Journal of Clinical and Translational Research

Journal homepage: http://www.jctres.com/en/home

ORIGINAL ARTICLE

Biocompatibility of calcitonin receptor fragment peptide-treated

3D-printed bone scaffolds: a muscle pouch implantation study

Vamiq M. Mustahsan Yanming Cai , David E. Komatsu , Imin Kao , Srinivas Pentyala *
1,3,
3
2
3
1,2
1 Department of Anesthesiology, Stony Brook University Renaissance School of Medicine, Stony Brook, 11794, NY, United States of America,
2 Department of Orthopaedics and Rehabilitation and Stony Brook University Renaissance School of Medicine, Stony Brook, 11794, NY, United States
of America, Department ofMechanical Engineering Stony Brook University College of Engineering and Applied Sciences, Stony Brook, 11794, NY,

3
United States of America
ARTICLE INFO ABSTRACT
Article history: Background and Aim: Current synthetic bone graft substitutes (BGSs) in development are limited by
Received: August 07, 2023 high resorption, poor load-bearing properties, and stress shielding. These limitations inhibit BGS from
Revised: September 26, 2023 complete biointegration. In this study, we developed calcitonin receptor fragment peptide (CRFP)-
Accepted: October 03, 2023 treated non-biodegradable MED610 scaffold, seeded with MC3T3 stem cells, and assessed their
Published online: November 18, 2023 in vivo biocompatibility and biointegration.
Methods: Scaffolds were fabricated with Stratasys MED610 (MED610) material, seeded with Mus
Keywords: musculus calvaria cells (MC3T3), and osteogenesis was induced with CRFP after the cells reached
Calcitonin receptor fragment peptide confluency and generated bone matrix. Scaffolds with and without bone matrix were implanted in
Osteogenic peptide male mice following a muscle pouch implantation protocol. Post-extraction, imaging, staining, and
ABS mechanical compression testing was carried after 3 weeks of scaffold implantation in the muscle to
MED610 measure the ectopic bone formation and compressive strength.
Scaffolds Results: The implanted scaffolds showed significantly higher (P < 0.01) calcium deposits in
3D printing comparison to the untreated scaffolds. We also found significantly higher (P < 0.001) mineralization
Muscle pouch on the implanted scaffolds compared to scaffolds before implantation. The mechanical properties of
the scaffolds did not vary significantly.
*Corresponding author: Conclusions: MED610 scaffolds treated with CRFP in vivo do not cause any adverse reaction when
Srinivas Pentyala implanted in muscle and showed significant ectopic bone formation, indicating biocompatibility and
Anesthesiology, HSC L4, Room: 060, bio-integration.
Renaissance School of Medicine, Stony Brook, Relevance for Patients: This study will aid in developing biomimetic and biocompatible artificial
NY, 11794-8480, United States of America. bones for implantation.
Tel: +1 631-444-2974
Fax: +1 631-444-2907
Email: Srinivas.pentyala@stonybrook.edu
1. Introduction
© 2023 Author(s). This is an Open-Access
article distributed under the terms of the In many orthopedic surgical procedures, metallic implants are used to fill in the defects
Creative Commons Attribution-Noncommercial formed due to surgery or fracture [1-3]. Metallic implants are strong and are able to withstand
License, permitting all non-commercial use, the load experienced by the bone. However, metallic implants lead to stress shielding and,
distribution, and reproduction in any medium, hence, weaken the surrounding bone [4]. Moreover, metallic implants are inert to bone
provided the original work is properly cited.
growth and inhibit complete implant integration [5]. The alternative for metallic implants
is biological implants vis, autografts and allografts [6]. Autografts are osteoconductive and
promote bio integration of the implant as they are extracted from the subject’s body [7].
They also have a lower rate of disease transmission in comparison to allografts. However,
autografts have other complications vis, lower availability, excessive pain, and increased
hospitalization cost due to extraction surgery [8,9]. Allografts exhibit reduced operating

DOI: http://dx.doi.org/10.18053/jctres.09.202306.23-00097