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Microbes & Immunity An ATP-free packaging of T4 DNA
DNA in a cell, but they need each other to accomplish Ethics approval and consent to participate
DNA packaging. When one end of DNA attaches or is
packaged into capsids by the motor-ATP system and the Not applicable.
ambient Pi concentration is reduced at the end of viral Consent for publication
42
reproduction or even after the burst of the host cell
(pfu of Figure 4D and E), the packaging of DNA will be Not applicable.
completed by the conformational change system. Because
the process of the conformational change system does not Availability of data
require extra ATP and proceeds automatically, it has been Data used in this work are available from the corresponding
“invisible” and might have been overlooked in previous author on reasonable request.
observations.
References
5. Conclusion
1. Birge EA. Bacterial and Bacteriophage Genetics. New York,
Motor-ATP system, the commonly known pathway, and USA: Springer Science-Business Media Inc.; 2010.
conformational change system, the new pathway, are the doi: 10.1007/0-387-31489-X
two genome packaging pathways. However, how these
two pathways play the different roles and cooperate in the 2. Gelbart WM, Knobler CM. Virology. Pressurized viruses.
process of DNA packaging remains elusive. Conventionally, Science. 2009;323:1682-1683.
cations were regarded as having the greatest effects on the doi: 10.1126/science.1170645
intracellular ionic conditions for viral infection. Anions, 3. Casjens S. The DNA-packaging nanomotor of tailed
especially phosphate, may receive more attention in this bacteriophages. Nat Rev Microbiol. 2011;9:647-657.
context. The direct observations of the amount of DNA doi: 10.1038/nrmicro2632
44
ejected by high Pi concentration and the connections
between the DNA-capsid and the DNA-tail by electron 4. Zhang H, Schwartz C, De Donatis GM, Guo P. “Push through
microscopy are anticipated. Further, research, for example, one-way valve’’ mechanism of viral DNA packaging. Adv
the experiment to investigate the conformational change Virus Res. 2012;83:415-465.
system in vivo, is necessary to elucidate the actual roles doi: 10.1016/B978-0-12-394438-2.00009-8
of these systems in the viral life cycle. The present study 5. Kottadiel VI, Rao VB, Chemla YR. The dynamic pause-
may potentially pave the way for the development of a new unpackaging state, an off-translocation recovery state of a
technology to transduce a large-size DNA into E. coli. DNA packaging motor from bacteriophage T4. Proc Nat
Acad Sci. 2012;109:20000-20005.
Acknowledgments
doi: 10.1073/pnas.1209214109
The author would like to acknowledge I. Koike for the
continuous scientific and social supports; H. Ogawa 6. Molineux IJ, Panja D. Popping the cork: Mechanisms of
phage genome ejection. Nat Rev Microbiol. 2013;11:194-204.
and H. Fukuda for funding acquisition, experimental
support and data analysis and interpretation; K. Kogure doi: 10.1038/nrmicro2988
for contributing to the discussion of results; Y. Fu for the 7. Effantin G, Boulanger P, Neumann E, Letellier L, Conway JF.
continuous support and help; and J. Furse and J. Adachi Bacteriophage T5 structure reveals similarities with HK97
for the assistance in data collection and in manuscript and T4 suggesting evolutionary relationships. J Mol Biol.
preparation. 2006;361:993-1002.
Funding doi: 10.1016/j.jmb.2006.06.081
8. Chow TY, Lin YT, Kuo TT. Stability of phage Xp12. Bot Bull
The research was supported by the Cooperative Program Acad Sinica. 1971;12:57-65.
(No. 119, 134, 2015) of Atmosphere and Ocean Research
Institute, the University of Tokyo to S.H. 9. Kuo TT, Huang TC, Wu RY, Chen CP. Specific dissociation of
phage Xpl2 by sodium citrate. J Gen Virol. 1971;10:199-202.
Conflict of interest doi: 10.1099/0022-1317-10-2-199
The author declares no conflicts of interest. 10. Shafia F, Thompson TL. Calcium ion requirement for
proliferation of bacteriophage PHI mu-4. J Bacteriol.
Author contributions 1964;88:293-296.
This is single-authored article. doi: 10.1128/jb.88.2.293-296.1964
Volume 1 Issue 1 (2024) 78 doi: 10.36922/mi.2666

