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Tumor Discovery                                                         Breast cancer optical differentiation




















































            Figure 2. The main block diagram of the two applied system approaches (reflection/transmission) to measure the tissue’s diffuse reflectance (R ) and light
                                                                                                      d
            transmission (T), which highlight the signal analysis for the optical properties and capture the spectral signatures of the investigated ex vivo breast tissue
                      r
            samples.
              Where     and       are the measured light intensity   It is essential to capture two data cubes each time a

            by R /T with the investigated breast tissue and the typical   breast sample is imaged: the first one is the dark data cube
                                                               which represents the image sensor’s dark current, and the
                  r
               d
            white plate reflection, respectively. The    demonstrates   second one is for a standard white reference for spectral
            the background light intensity discriminated  by R /T  r  calibration . First, the acquired dark cube was taken by
                                                                       [56]
                                                       d
            without the sample reflection on the plate.        closing the lens of the HS camera with its cap. The data
                                                               of the dark and white cubes were exploited to adjust the
            2.6. Spectral arrangement                          reflected sample image to remove the noise impacts of the
            The hyper spectral images were taken for every investigated   investigated sample tissue, as expressed in Equation IX:
            breast tissue sample from the two different arrangements
            as explained previously with spatial pixels (max/                                             (IX)
            nominal) = 1040/520, spectral channels = 128, HS cube
            captured 100 frames/s within 6.96  s/cube. They applied   Where    is the relative reflectance of the
            statistical analysis for the spectral signature for selecting   investigated sample image,    is the seized image,
            the optimum spectral range to discriminate the normal    is the opaque image cube, and    is the attained
            from the abnormal tissue.                          image of the white reflectance board.



            Volume 2 Issue 1 (2023)                         6                           https://doi.org/10.36922/td.258
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