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Tumor Discovery CK2 deregulation in non-small-cell lung cancer

A

B

C

Figure 5. Immunohistochemical detection of CK2 subunits and CK2 substrate phosphorylation (AKT) in LUAD specimens and para-neoplastic tissues.
Representative staining results for each CK2 subunit are shown: (A) in tumor and (B) in para-neoplastic tissues as a brown color; (C) Total AKT and
phosphorylated AKTs129 (pAKT) protein levels were measured in tumor (LUAD) and para-neoplastic (Para-) tissues as an indicator of CK2 enzymatic
activity in situ. Images were captured at 200× magnification. Scale bars: 100 μm.
Abbreviations: LUAD: Lung adenocarcinoma; CK2: Casein kinase-2.

Table 1. Immunohistochemical detection and quantification using a combined immunoreactivity score for independent CK2
subunits in primary tumor specimens of NSCLC and para-neoplastic tissues
Groups Sample size Score Positive rate z-score P-value
− + ++ +++
CK2A1
NSCLC 134 30 50 27 27 77.6 −9.864 0.000
Para-neoplastic 134 107 24 2 1 20.1 0.000
CK2A2
NSCLC 134 16 55 41 21 88.1 −7.248 0.000
Para-neoplastic 134 65 49 16 4 51.5 0.000
CK2B
NSCLC 134 13 48 35 38 90.3 −8.538 0.000
Para-neoplastic 134 76 35 18 5 43.3 0.000
Ki-67
NSCLC 134 58 32 20 24 56.7 −9.722 0.000
Para-neoplastic 134 131 3 0 0 2.2 0.000
Notes: Score 1 – 3: − (negative); Score 4 – 5: + (weak positive); Score 6 – 7: ++ (positive); Score ≥8: +++ (strong positive); Two-sided P<0.05 was
considered statistically significant for all calculations.
Abbreviation: NSCLC: Non-small-cell lung cancer.

2/2, and 3/3 significant unique analyses, respectively, 16 additional tumor types indicated cancer-type-specific
involving lung cancer specimens. In addition, Kaplan– up- or downregulation of CSNK2 transcripts, as well as
Meier analysis revealed a significant correlation between direct and inverse correlations between CSNK2 transcripts
CSNK2B overexpression and reduced OS, while CSNK2A2 and OS. Overall, the prevailing notion is that CK2’s
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overexpression was unexpectedly associated with aberrant expression levels or activity are instrumental to
improved OS in LUAD. A follow-up analysis analyzing malignant transformation and promotion; however, the
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Volume 3 Issue 4 (2024) 9 doi: 10.36922/td.4571