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Advanced Neurology

ORIGINAL RESEARCH ARTICLE
AT receptor role in the hypothalamic and renal
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function interaction

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2
Celia Ruberto , Victoria Belén Occhieppo , Claudia Bregonzio *, and
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Gustavo Baiardi 1
1 Laboratory of Neuropharmacology, IIBYT-CONICET, National University of Córdoba, Córdoba,
Argentina
2 Experimental Pharmacology Institute of Córdoba, IFEC-CONICET, Department of Pharmacology
“Otto Orsingher,” Faculty of Chemical Sciences, National University of Córdoba, Córdoba, Argentina

Abstract

Angiotensin II (ANG II) is involved in renal sodium homeostasis under normal and
pathological conditions in close relation with the sympathetic nervous system.
Vasopressin, a hormone that modulates renal sodium and water reabsorption, is
synthesized and released in the supraoptic and paraventricular nucleus under the
influence of ANG II. We hypothesized that brain ANG II (AT ) receptors regulate renal
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sodium and water reabsorption and excretion through the sympathetic nervous
system. In this study, male Wistar rats with renal denervation/sham were fed a
hypersodic (4%) or normal (0.4%) diet and evaluated during 5 days in metabolic
cages. On day 5, they were injected in the lateral ventricle with an AT receptor
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antagonist, losartan, and sacrificed 12 h later; blood samples and brains were
obtained for evaluation. The urine was collected daily. The neuronal activation was
analyzed in the nucleus of the supraoptic, paraventricular, subfornical, and organum
*Corresponding author: vasculosum of the lamina terminalis. Activation of vasopressin neurons was evaluated
Claudia Bregonzio in the supraoptic nucleus. Depending on renal nerve integrity, the hypersodic
(claubregonzio@unc.edu.ar)
diet or losartan administration differentially affected neuronal activation. In sham
Citation: Ruberto C, animals, losartan prevented the stimulatory effects induced by the hypersodic diet
Occhieppo VB, Bregonzio C,
et al., 2023, AT receptor role in in water intake and the neuronal activation in vasopressin-positive neurons. Renal
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the hypothalamic and renal function denervation modified the effect of the hypersodic diet on water intake, urinary
interaction. Adv Neuro, 2(2): 393. volume, and creatinine excretion, and losartan administration was able to prevent
https://doi.org/10.36922/an.393 these alterations. Food intake was similar in all groups. Our results suggest that brain
Received: March 22, 2023 AT receptors regulate renal sodium and water reabsorption through the sympathetic
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Accepted: May 2, 2023 nervous system in close interaction with vasopressin.
Published Online: May 24, 2023
Keywords: Renal denervation; Kidney; Vasopressin; Losartan; Sodium intake
Copyright: © 2023 Author(s).
This is an Open-Access article
distributed under the terms of the
Creative Commons Attribution
License, permitting distribution, 1. Introduction
and reproduction in any medium,
provided the original work is Brain angiotensin II (ANG II) action through AT receptors (AT -R) is involved in the
properly cited. 1 1
regulation of cerebral blood flow, body fluids, and mineral balance and osmoregulatory
Publisher’s Note: AccScience homeostasis-associated behaviors such as thirst and sodium intake [1-4] . ANG II is
Publishing remains neutral with
regard to jurisdictional claims in a neuropeptide that when activating AT -R, plays a key role in the regulation of the
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published maps and institutional sympathetic and neuroendocrine systems . Moreover, peripheral ANG II acting
[5]
affiliations.
Volume 2 Issue 2 (2023) 1 https://doi.org/10.36922/an.393