Page 57 - AN-2-4

Advanced Neurology Genomic insights into Alzheimer

Table 2. Linear and discontinued B‑cell epitopes prediction from the 3D structure of the wild‑type and 20 mutated human APP
Mutation type Linear B‑cell Linear B‑cell Discontinuous B‑cell Total
epitope by Ellipro epitope by Bepipred epitopes by Ellipro
Wild-type APP 11 19 6 36
KM670/671NL (Swe) 12 19 5 36
A673V 13 19 6 38
D678N (Tot) 11 21 5 37
D678H (Tai) 12 17 12 41
A692G (Fle) 12 19 7 38
E693Del (Osa) 11 21 4 36
E693G (Arctic) 14 19 7 40
D694N (Iowa) 11 19 5 35
T714A (Ira) 11 19 6 36
T714I (Austrian) 12 20 5 37
V715A (Ger) 13 20 4 37
V715M (Fre) 13 19 5 37
I716F (Ibe) 12 19 5 36
V717F (Ind) 12 18 5 35
V717G 11 20 6 37
V717I (Lon) 10 19 4 33
V717L 14 19 7 40
T719N 12 20 7 39
M722K 12 19 7 38
L723P (Australian) 14 20 6 40
Abbreviation: APP: Amyloid precursor protein.

Table 3. Common toxic linear B‑cell epitopes from the 3D structure of the human wild type and mutated APP

Common toxic B‑cell epitopes Position Mutation subtypes
GVEFVCCPLAEES 181 – 193 I716F, V717I, A692G, V717G, T719N
DAEEDDSDVWW 200 – 210 I716V, I716T, A673V, E682K, E693G
SDPSGTKTCIDTKEGILQYCQ 54 – 74 A673V, V717L, T719P, KM670/671NL
IQNWCKRGRKQCKTHPHFVIPYRCLV 94 – 119 APP, V717F, I716F, V717I, A692G, M722K
YCQEVYPELQITNVVEANQP 72 – 91 L723P, K724N, V715M, I716M, I716F, T714A, M722K, T719N,
T719P, D678H, V717L, E693Del, D678N
CKRGRKQCKT 98 – 107 APP, L723P, K724N, I716V, V715M, V715A, I716M, I716T, I716F,
V717F, T714A, V717I, M722K, KM670/671NL, T719N, T719P,
D678H, V717G, V717L, A673V, E693Del, D678N
DKFRGV 177 – 182 APP, I716V, I716F, V717I, E693Del, D678N
CSEKSTNLH 158 – 166 T714I, V717F, E682K, V717G, A692G
DKFRGVEF 177 – 184 K724N, V715M, M722K, T719N, T719P, V717L
CGGNRNNFDTE 324 – 334 APP, T714I, K724N, I716V, V715M, V715A, I716M, I716T, I716F,
V717F, V717I, V717F, E682K, L723P, M722K, T719N, T719P, V717G,
V717L, A673V, A692G, D694N, D678N
AAVTPEERHLSKMQQNGYENPTYKFFE 740 – 766 APP, L723P, T714I, K724N, I716V, V715M, V715A, I716M, I716T,
I716F, V717F, V717I, V717F, T714A, E682K, M722K, KM670/671NL,
T719N, T719P, D678H, V717G, V717L, A673V, A692G, D694N,
D678N
Abbreviation: APP: Amyloid precursor protein.

Volume 2 Issue 4 (2023) 12 https://doi.org/10.36922/an.1734