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Gene & Protein in Disease                      DNA methylation and gene expression on rats with protein malnutrition













































            Figure 1. Regional distribution of reference genome alignment.

            Table 3. Statistical table of gene expression value distribution in each sample.

             Sample     Exp gene   Min.    1  Qu.   Median     Mean     3  Qu.     Max.       Sd.       Sum.
                                                                         rd
                                            st
            LPE_1        13,897    0.00     0.17     0.68      20.92     2.12    100,118.16  944.65   290,677.27
            LPE_2        14,566    0.00     0.25     1.20      20.94     3.83     79,119.20  782.82   304,980.25
            LPE_3        12,791    0.00     0.09     0.28      22.73     0.93    103,934.75  1047.15  290,738.28
            LPE_4        13,908    0.00     0.22     0.88      21.48     2.70     95,642.32  912.56   298,781.54
            LPF_1        12,113    0.00     0.07     0.21      24.51     0.73    124,902.09  1245.18  296,859.96
            LPF_2        13,894    0.00     0.19     0.75      21.24     2.25     90,630.45  899.69   295,102.61
            LPF_3        13,937    0.00     0.17     0.71      22.50     2.17    114,011.71  1079.30  313,581.54
            LPF_4        14,273    0.00     0.23     0.96      22.13     2.89    107,773.62  977.68   315,828.50
            CON_1        14,332    0.00     0.22     1.03      21.81     3.28    110,772.82  998.48   312,629.31
            CON_2        13,366    0.00     0.14     0.50      20.85     1.49     98,315.52  965.55   278,649.78
            CON_3        13,951    0.00     0.20     0.85      20.87     2.54     96,050.62  922.73   291,113.76
            CON_4        15,626    0.00     0.38     2.78      21.20     9.32     49,694.29  473.42   331,285.81
            LPE: Early-life low-protein group, LPF: Fetal low-protein group, CON: Control group

            of gene transcripts of each sample was measured by the   the  change  of  expression  trend  among  different  samples
            concept of coverage (Table 5).                     (Figure  3). Ideally, the expression density map of each
              The expression density map of log10 (FPKM) after the   sample should conform to the normal distribution, and
            treatment of different samples  can be  used to  compare   the expression trend of biological repeat samples should


            Volume 1 Issue 2 (2022)                         6                      https://doi.org/10.36922/gpd.v1i2.169
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